Key features and details
- Mouse monoclonal [SM23/496] to Sumo 2 + Sumo 3
- Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cyt, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Sumo 2 + Sumo 3 antibody [SM23/496]
See all Sumo 2 + Sumo 3 primary antibodies
DescriptionMouse monoclonal [SM23/496] to Sumo 2 + Sumo 3
Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-P, Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
- HeLa cells; breast carcinoma and tonsil tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
Purification notes200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G
Light chain typekappa
Our Abpromise guarantee covers the use of ab190757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IP||Use at 1-2 µg/mg of lysate.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11,13 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
RelevanceSUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cellular localizationCytoplasmic (SUMO3) and Nuclear (SUMO2)
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
ab190757 has not yet been referenced specifically in any publications.