Overview

  • Product name

    Anti-Sumo 2 antibody [SPM621] - BSA and Azide free
    See all Sumo 2 primary antibodies
  • Description

    Mouse monoclonal [SPM621] to Sumo 2 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Sumo 2.
    Database link: P55854

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab212838 was purified from bioreactor concentrate by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SPM621
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
  • Tissue specificity

    Broadly expressed.
  • Sequence similarities

    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications

    Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4.
    Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
  • Cellular localization

    Nucleus. Nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSMT 3 antibody
    • HSMT3 antibody
    • MGC117191 antibody
    • Sentrin 2 antibody
    • Sentrin-2 antibody
    • Sentrin2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin related modifier 2 antibody
    • Small ubiquitin-related modifier 2 antibody
    • SMT 3B antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3, yeast, homolog 2 antibody
    • Smt3A antibody
    • SMT3B antibody
    • SMT3H2 antibody
    • SUMO-2 antibody
    • SUMO-3 antibody
    • SUMO2 antibody
    • SUMO2_HUMAN antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3B antibody
    • Ubiquitin-like protein SMT3A antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Sumo 2 using ab212838 at 1 µg/ml in immnohistochemical analysis.

    This IHC-P data was generated using the same anti-Sumo 2 antibody clone, SPM621, in a different buffer formulation.

References

ab212838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab212838.
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