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Rabbit monoclonal [EPR300(2)] to Sumo 2+3
ICC more details Unsuitable for:
Flow Cyt or IP
Synthetic peptide within Human Sumo 2+3 aa 1-100. The exact sequence is proprietary.
Paraffin-embedded Human ovarian tissue
HeLa and Jurkat cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.
Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cytoplasmic (SUMO3) and Nuclear (SUMO2)
Western blot - Anti-Sumo 2+3 antibody [EPR300(2)] (ab109196)
All lanes : Anti-Sumo 2+3 antibody [EPR300(2)] (ab109196) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2+3 antibody [EPR300(2)] (ab109196)
ab109196, at 1/100, staining Sumo 2+3 in paraffin-embedded Human ovarian tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"