Recombinant
RabMAb

Anti-Sumo 2+3 antibody [EPR4602] (ab109005)

Overview

  • Product name
    Anti-Sumo 2+3 antibody [EPR4602]
    See all Sumo 2+3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4602] to Sumo 2+3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Sumo 2+3 aa 1 to the C-terminus.

  • Positive control
    • HeLa, Jurkat, 293T, SW480 and HL60 cell lysates; Human colonic and hepatocellular carcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4602
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/400.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
    • Cellular localization
      Cytoplasmic (SUMO3) and Nuclear (SUMO2)
    • Database links
    • Alternative names
      • HSMT3 antibody
      • MGC117191 antibody
      • OTTHUMP00000115275 antibody
      • OTTHUMP00000115276 antibody
      • OTTHUMP00000115277 antibody
      • Sentrin 2 antibody
      • Small ubiquitin like modifier 2 antibody
      • Small ubiquitin related modifier 2 antibody
      • small ubiquitin-like modifier 3 antibody
      • small ubiquitin-related modifier 3 antibody
      • SMT3 homolog 1 antibody
      • SMT3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 1 antibody
      • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 3 antibody
      • SMT3A antibody
      • SMT3B antibody
      • SMT3H1 antibody
      • SMT3H2 antibody
      • Sumo2 antibody
      • Sumo3 antibody
      • Ubiquitin like protein SMT3A antibody
      • Ubiquitin like protein SMT3B antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Sumo 2+3 antibody [EPR4602] (ab109005) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SW480 cell lysate
      Lane 5 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa

    • ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    References

    This product has been referenced in:
    • Schilling EM  et al. The Human Cytomegalovirus IE1 Protein Antagonizes PML Nuclear Body-Mediated Intrinsic Immunity via the Inhibition of PML De Novo SUMOylation. J Virol 91:N/A (2017). Read more (PubMed: 27903803) »
    • Bogachek MV  et al. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports 7:1140-1151 (2016). Read more (PubMed: 27916539) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (RPE)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    RPE
    Blocking step
    Milk (left) and BSA (right) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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