Overview

  • Product name

    Anti-Sumo 3 antibody [1H9L17]
    See all Sumo 3 primary antibodies
  • Description

    Rabbit monoclonal [1H9L17] to Sumo 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Sumo 3 aa 91-103.
    Sequence:

    GGVPESSLAGHSF


    Database link: P55854

  • Positive control

    • Jurkat and U2OS cells; Human colon carcinoma tissue; Mouse brain, testis and lung tissue lysates; Rat brain and testis tissue lysates; PC12 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1H9L17
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 4 - 6 µg/ml.
Flow Cyt Use at an assay dependent concentration.

Use at 0.5-1 µg/test

IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 2 - 3 µg/ml. Predicted molecular weight: 11 kDa.

Target

  • Function

    Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
  • Tissue specificity

    Expressed predominantly in liver.
  • Sequence similarities

    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications

    Polymeric chains can be formed through Lys-11 cross-linking.
    Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Small ubiquitin like modifier 3 antibody
    • Small ubiquitin related modifier 3 antibody
    • Small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3, yeast, homolog 1 antibody
    • SMT3A antibody
    • Smt3B antibody
    • SMT3H1 antibody
    • SUMO-2 antibody
    • SUMO-3 antibody
    • sumo3 antibody
    • SUMO3_HUMAN antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin-like protein SMT3B antibody
    see all

Images

  • All lanes : Anti-Sumo 3 antibody [1H9L17] (ab203570) at 2 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat testis tissue lysate

    Predicted band size: 11 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling Sumo 3 using ab203570 at 5 μg/mL, followed by DAB detection.

  • Immunofluorescent analysis of U2OS cells labeling Sumo 3 using ab203570 at 5 μg/mL (left image). Right image: sample with immunogenic phosphopeptide. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody.

  • Flow cytometric analysis of fixed and permealized Jurkat cells labeling Sumo 3 using ab203570 at 0.5 μg/test (Black) followed by secondary detection with Alexa Fluor® 488 Goat anti-Rabbit. Cells incubated without primary antibody (grey) for control. Preincubation with immunizing peptide decreased the staining (red). 

References

ab203570 has not yet been referenced specifically in any publications.

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