Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [1H9L17] to Sumo 3
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Sumo 3 antibody [1H9L17]
See all Sumo 3 primary antibodies
DescriptionRabbit monoclonal [1H9L17] to Sumo 3
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Jurkat and U2OS cells; Human colon carcinoma tissue; Mouse brain, testis and lung tissue lysates; Rat brain and testis tissue lysates; PC12 cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 4 - 6 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
Use at 0.5-1 µg/test
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||Use a concentration of 2 - 3 µg/ml. Predicted molecular weight: 11 kDa.|
FunctionUbiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
Tissue specificityExpressed predominantly in liver.
Sequence similaritiesBelongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.
modificationsPolymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
- Information by UniProt
- Small ubiquitin like modifier 3 antibody
- Small ubiquitin related modifier 3 antibody
- Small ubiquitin-related modifier 3 antibody
All lanes : Anti-Sumo 3 antibody [1H9L17] (ab203570) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : PC12 cell lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat testis tissue lysate
Predicted band size: 11 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling Sumo 3 using ab203570 at 5 μg/mL, followed by DAB detection.
Immunofluorescent analysis of U2OS cells labeling Sumo 3 using ab203570 at 5 μg/mL (left image). Right image: sample with immunogenic phosphopeptide. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody.
Flow cytometric analysis of fixed and permealized Jurkat cells labeling Sumo 3 using ab203570 at 0.5 μg/test (Black) followed by secondary detection with Alexa Fluor® 488 Goat anti-Rabbit. Cells incubated without primary antibody (grey) for control. Preincubation with immunizing peptide decreased the staining (red).
ab203570 has not yet been referenced specifically in any publications.