Recombinant
Recombinant Anti-SUN1 antibody [EPR6554] (ab124770)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-SUN1 antibody [EPR6554]
See all SUN1 primary antibodies -
Description
Rabbit monoclonal [EPR6554] to SUN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human SUN1 aa 100-200.
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Positive control
- Ramos, K562, HeLa, Jurkat and A431 cell lysates; Human ovary carcinoma tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6554 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100 - 1/250. |
Application notes
Is unsuitable for IP.
Target
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Function
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs). -
Sequence similarities
Contains 1 SUN domain. -
Domain
The SUN domain may play a role in the nuclear anchoring and/or migration. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 23353 Human
- Omim: 607723 Human
- SwissProt: O94901 Human
- Unigene: 438072 Human
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Alternative names
- Protein unc-84 homolog A antibody
- Sad1 and UNC84 domain containing 1 antibody
- Sad1/unc 84 protein like 1 antibody
see all
Images
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![Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)](https://www.abcam.com/ps/products/124/ab124770/Images/ab124770-171565-anti-sun1-antibody-epr6554-western-blot.png)
Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)This image is courtesy of an anonymous abreview. -
![Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)](https://www.abcam.com/ps/products/124/ab124770/Images/ab124770-138633-anti-sun1-antibody-epr6554-western-blot.jpg)
Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)](https://www.abcam.com/ps/products/124/ab124770/Images/ab124770-138632-anti-sun1-antibody-epr6554-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.
Protocols
References
This product has been referenced in:
- Pappas SS et al. TorsinA dysfunction causes persistent neuronal nuclear pore defects. Hum Mol Genet 27:407-420 (2018). Read more (PubMed: 29186574) »
- Huang W et al. SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma. Onco Targets Ther 10:2825-2833 (2017). WB ; Human . Read more (PubMed: 28652764) »
