Product nameAnti-SUN1 antibody [EPR6554]
See all SUN1 primary antibodies
DescriptionRabbit monoclonal [EPR6554] to SUN1
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide corresponding to Human SUN1 aa 100-200.
- Ramos, K562, HeLa, Jurkat and A431 cell lysates; Human ovary carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
Sequence similaritiesContains 1 SUN domain.
DomainThe SUN domain may play a role in the nuclear anchoring and/or migration.
Cellular localizationNucleus inner membrane.
- Information by UniProt
- Protein unc-84 homolog A antibody
- Sad1 and UNC84 domain containing 1 antibody
- Sad1/unc 84 protein like 1 antibody
All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.
This product has been referenced in:
- Pappas SS et al. TorsinA dysfunction causes persistent neuronal nuclear pore defects. Hum Mol Genet 27:407-420 (2018). Read more (PubMed: 29186574) »
- Huang W et al. SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma. Onco Targets Ther 10:2825-2833 (2017). WB ; Human . Read more (PubMed: 28652764) »