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  1. Link

    sun1-antibody-epr6554-ab124770.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nuclear Envelope
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RecombinantRabMAb

Recombinant Anti-SUN1 antibody [EPR6554] (ab124770)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (10)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
  • Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
  • Anti-SUN1 antibody [EPR6554] (ab124770)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6554] to SUN1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-SUN2 antibody [EPR6557] (ab124916)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free (ab215967)

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Overview

  • Product name

    Anti-SUN1 antibody [EPR6554]
    See all SUN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6554] to SUN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SUN1 aa 100-200.

  • Positive control

    • IHC-P: Human ovary carcinoma and human breast tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6554
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Alternative Versions

    • Anti-SUN1 antibody [EPR6554] - BSA and Azide free (ab226091)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 nuclear extract lysate (ab14851)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

See IHC antigen retrieval protocols.

Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
  • Sequence similarities

    Contains 1 SUN domain.
  • Domain

    The SUN domain may play a role in the nuclear anchoring and/or migration.
  • Cellular localization

    Nucleus inner membrane.
  • Target information above from: UniProt accession O94901 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23353 Human
    • Omim: 607723 Human
    • SwissProt: O94901 Human
    • Unigene: 438072 Human
    • Alternative names

      • Protein unc-84 homolog A antibody
      • Sad1 and UNC84 domain containing 1 antibody
      • Sad1/unc 84 protein like 1 antibody
      • Sad1/unc-84 protein-like 1 antibody
      • Sun domain-containing protein 1 antibody
      • Sun1 antibody
      • SUN1_HUMAN antibody
      • UNC 84 homolog A (C elegans) antibody
      • UNC 84A antibody
      • UNC84 A antibody
      • UNC84 antibody
      • UNC84 homolog A (C elegans) antibody
      • UNC84A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SUN1 with purified ab124770 at 1/200 dilution (0.698 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)
      Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)
      All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/5000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 90 kDa
      Observed band size: 100,90 kDa why is the actual band size different from the predicted?



      The bands observed are consistent with what has been described in PMID: 16648470

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)

      Unpurified ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-SUN1 antibody [EPR6554] (ab124770)
      Anti-SUN1 antibody [EPR6554] (ab124770)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab124770? Please let us know so that we can cite the reference in this datasheet.

    ab124770 has been referenced in 10 publications.

    • Carley E  et al. The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation. Elife 10:N/A (2021). PubMed: 33779546
    • Yang CW  et al. Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells. Aging (Albany NY) 13:10490-10516 (2021). PubMed: 33820871
    • Bikkul MU  et al. Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform. Genes Chromosomes Cancer 58:341-356 (2019). PubMed: 30474255
    • Mistriotis P  et al. Confinement hinders motility by inducing RhoA-mediated nuclear influx, volume expansion, and blebbing. J Cell Biol 218:4093-4111 (2019). PubMed: 31690619
    • Reddy K  et al. A CTG repeat-selective chemical screen identifies microtubule inhibitors as selective modulators of toxic CUG RNA levels. Proc Natl Acad Sci U S A 116:20991-21000 (2019). PubMed: 31570586
    • Tan KL  et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell 45:226-244.e8 (2018). PubMed: 29689197
    • Pappas SS  et al. TorsinA dysfunction causes persistent neuronal nuclear pore defects. Hum Mol Genet 27:407-420 (2018). PubMed: 29186574
    • Huang W  et al. SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma. Onco Targets Ther 10:2825-2833 (2017). WB ; Human . PubMed: 28652764
    • Stewart RM  et al. Nuclear-cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. J Cell Biol 209:403-18 (2015). PubMed: 25963820
    • Chow KH  et al. The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Mol Biol Cell 25:160-8 (2014). Human . PubMed: 24196834

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-SUN1 antibody [EPR6554]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 04 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SUN1 antibody [EPR6554]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MC3T3 cells)
    Permeabilization
    Yes - NP40
    Specification
    MC3T3 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 13 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SUN1 antibody [EPR6554]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 15 2018

    Western blot

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    36 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification
    HeLa, RPE-1
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 03 2013

    Western blot abreview for Anti-SUN1 antibody [EPR6554]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (skin)
    Loading amount
    10 µg
    Specification
    skin
    Treatment
    SiRNA SUN1for 48h
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 10 2012

    Western blot abreview for Anti-SUN1 antibody [EPR6554]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Loading amount
    20 µg
    Specification
    U2OS
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    DR. Andrew Cobb

    Verified customer

    Submitted Jul 11 2012

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