Recombinant Anti-SUN1 antibody [EPR6554] (ab124770)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-SUN1 antibody [EPR6554]
See all SUN1 primary antibodies -
Description
Rabbit monoclonal [EPR6554] to SUN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human SUN1 aa 100-200.
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Positive control
- IHC-P: Human ovary carcinoma and human breast tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6554 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs). -
Sequence similarities
Contains 1 SUN domain. -
Domain
The SUN domain may play a role in the nuclear anchoring and/or migration. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 23353 Human
- Omim: 607723 Human
- SwissProt: O94901 Human
- Unigene: 438072 Human
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Alternative names
- Protein unc-84 homolog A antibody
- Sad1 and UNC84 domain containing 1 antibody
- Sad1/unc 84 protein like 1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)](https://www.abcam.com/ps/products/124/ab124770/Images/ab124770-4-anti-sun1-antibody-epr6554-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SUN1 with purified ab124770 at 1/200 dilution (0.698 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)](https://www.abcam.com/ps/products/124/ab124770/Images/ab124770-365473-anti-sun1-antibody-epr6554-westernblot-human.jpg)
Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 100,90 kDa why is the actual band size different from the predicted?The bands observed are consistent with what has been described in PMID: 16648470
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)](https://www.abcam.com/ps/products/124/ab124770/Images/ab124770-138632-anti-sun1-antibody-epr6554-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)Unpurified ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
References
This product has been referenced in:
- Bikkul MU et al. Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform. Genes Chromosomes Cancer 58:341-356 (2019). Read more (PubMed: 30474255) »
- Tan KL et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell 45:226-244.e8 (2018). Read more (PubMed: 29689197) »
