Overview

  • Product name
    Anti-SUN1 antibody [EPR6554]
    See all SUN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6554] to SUN1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SUN1 aa 100-200.

  • Positive control
    • Ramos, K562, HeLa, Jurkat and A431 cell lysates; Human ovary carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
    • Sequence similarities
      Contains 1 SUN domain.
    • Domain
      The SUN domain may play a role in the nuclear anchoring and/or migration.
    • Cellular localization
      Nucleus inner membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • Protein unc-84 homolog A antibody
        • Sad1 and UNC84 domain containing 1 antibody
        • Sad1/unc 84 protein like 1 antibody
        • Sad1/unc-84 protein-like 1 antibody
        • Sun domain-containing protein 1 antibody
        • Sun1 antibody
        • SUN1_HUMAN antibody
        • UNC 84 homolog A (C elegans) antibody
        • UNC 84A antibody
        • UNC84 A antibody
        • UNC84 antibody
        • UNC84 homolog A (C elegans) antibody
        • UNC84A antibody
        see all

      Images

      • Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)
        Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)This image is courtesy of an anonymous abreview.

        See Abreview

      • Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)
        Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)
        All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/1000 dilution

        Lane 1 : Ramos cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : Jurkat cell lysate
        Lane 5 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 90 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)
        ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

      References

      This product has been referenced in:
      • Huang W  et al. SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma. Onco Targets Ther 10:2825-2833 (2017). WB ; Human . Read more (PubMed: 28652764) »
      • Stewart RM  et al. Nuclear-cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. J Cell Biol 209:403-18 (2015). Read more (PubMed: 25963820) »

      See all 3 Publications for this product

      Publishing research using ab124770? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      4 reviews or Q&A

      10
      Votes: Highest First

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SUN1 antibody [EPR6554]

       Excellent
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Vascular smooth muscle cells)
      Permeabilization
      Yes - NP40
      Specification
      Vascular smooth muscle cells
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Aug 15 2018

      Western blot

       Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Loading amount
      36 µg
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Sample
      Human Cell lysate - whole cell (HeLa, RPE-1)
      Specification
      HeLa, RPE-1
      Blocking step
      Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted May 03 2013

      Western blot abreview for Anti-SUN1 antibody [EPR6554]

       Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (skin)
      Loading amount
      10 µg
      Specification
      skin
      Treatment
      SiRNA SUN1for 48h
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Aug 10 2012

      Western blot abreview for Anti-SUN1 antibody [EPR6554]

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS)
      Loading amount
      20 µg
      Specification
      U2OS
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      abreview image
      Username

      Dr. Andrew Cobb

      Verified customer

      Submitted Jul 11 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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