Overview

  • Product name

    Anti-SUN1 antibody [EPR6554]
    See all SUN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6554] to SUN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SUN1 aa 100-200.

  • Positive control

    • Ramos, K562, HeLa, Jurkat and A431 cell lysates; Human ovary carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
    • Sequence similarities

      Contains 1 SUN domain.
    • Domain

      The SUN domain may play a role in the nuclear anchoring and/or migration.
    • Cellular localization

      Nucleus inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Protein unc-84 homolog A antibody
      • Sad1 and UNC84 domain containing 1 antibody
      • Sad1/unc 84 protein like 1 antibody
      • Sad1/unc-84 protein-like 1 antibody
      • Sun domain-containing protein 1 antibody
      • Sun1 antibody
      • SUN1_HUMAN antibody
      • UNC 84 homolog A (C elegans) antibody
      • UNC 84A antibody
      • UNC84 A antibody
      • UNC84 antibody
      • UNC84 homolog A (C elegans) antibody
      • UNC84A antibody
      see all

    Images

    • All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 90 kDa

    • ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Pappas SS  et al. TorsinA dysfunction causes persistent neuronal nuclear pore defects. Hum Mol Genet 27:407-420 (2018). Read more (PubMed: 29186574) »
    • Huang W  et al. SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma. Onco Targets Ther 10:2825-2833 (2017). WB ; Human . Read more (PubMed: 28652764) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 15 2018

    Western blot

    Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    36 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification
    HeLa, RPE-1
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted May 03 2013

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (skin)
    Loading amount
    10 µg
    Specification
    skin
    Treatment
    SiRNA SUN1for 48h
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 10 2012

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Loading amount
    20 µg
    Specification
    U2OS
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. Andrew Cobb

    Verified customer

    Submitted Jul 11 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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