Recombinant
RabMAb

Recombinant Anti-SUN1 antibody [EPR6554] - BSA and Azide free (ab226091)

Overview

  • Product name

    Anti-SUN1 antibody [EPR6554] - BSA and Azide free
    See all SUN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6554] to SUN1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SUN1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Human ovary carcinoma and human breast tissues.
  • General notes

    Ab226091 is the carrier-free version of ab124770. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226091 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

WB Use at an assay dependent concentration. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
    • Sequence similarities

      Contains 1 SUN domain.
    • Domain

      The SUN domain may play a role in the nuclear anchoring and/or migration.
    • Cellular localization

      Nucleus inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Protein unc-84 homolog A antibody
      • Sad1 and UNC84 domain containing 1 antibody
      • Sad1/unc 84 protein like 1 antibody
      • Sad1/unc-84 protein-like 1 antibody
      • Sun domain-containing protein 1 antibody
      • Sun1 antibody
      • SUN1_HUMAN antibody
      • UNC 84 homolog A (C elegans) antibody
      • UNC 84A antibody
      • UNC84 A antibody
      • UNC84 antibody
      • UNC84 homolog A (C elegans) antibody
      • UNC84A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SUN1 with purified ab124770 at 1/200 dilution (0.698 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124770)
    • ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124770).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab226091 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226091.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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