Overview

  • Product name

    Anti-SUN2 antibody [EPR6556]
    See all SUN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6556] to SUN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SUN2 aa 250-350 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Human fetal muscle tissue lysate; Saos-2, HeLa and HepG2 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Relevance

      SUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
    • Cellular localization

      Nuclear membrane, endosome membrane, mitotic spindle organization.
    • Database links

    • Alternative names

      • FRIGG antibody
      • KIAA0668 antibody
      • nuclear envelope protein antibody
      • Protein unc-84 homolog B antibody
      • Rab5 interacting protein antibody
      • RAB5IP antibody
      • Sad1 and UNC84 domain containing 2 antibody
      • Sad1 unc-84 domain protein 2 antibody
      • Sad1 unc84 domain protein antibody
      • Sad1/unc-84 protein-like 2 antibody
      • Sad1/unc84 protein-like antibody
      • SUN domain-containing protein 2 antibody
      • UNC 84B antibody
      • unc84 homolog B (C. elegans) antibody
      • unc84 homolog B antibody
      • unc84, C. elegans, homolog of, B antibody
      • UNC84B antibody
      see all

    Images

    • All lanes : Anti-SUN2 antibody [EPR6556] (ab133591) at 1/1000 dilution

      Lane 1 : Human fetal muscle tissue lysate
      Lane 2 : Saos 2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 80 kDa

    • ab133591 at 1/30 dilution immunoprecipitating SUN2 in Saos-2 (human osteosarcoma) cell lysate. Total sample is 300µg. The large spread out band at 50 kDa is Rabbit IgG heavy chain.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Sun WW  et al. SUN2 Modulates HIV-1 Infection and Latency through Association with Lamin A/C To Maintain the Repressive Chromatin. MBio 9:N/A (2018). Read more (PubMed: 29717016) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133591.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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