Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6556] to SUN2
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-SUN2 antibody [EPR6556]
See all SUN2 primary antibodies
DescriptionRabbit monoclonal [EPR6556] to SUN2
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human SUN2 aa 250-350 (internal sequence). The exact sequence is proprietary.
- Human fetal muscle tissue lysate; Saos-2, HeLa and HepG2 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.01 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 80 kDa.|
|IP||1/10 - 1/100.|
RelevanceSUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
Cellular localizationNuclear membrane, endosome membrane, mitotic spindle organization.
- FRIGG antibody
- KIAA0668 antibody
- nuclear envelope protein antibody
All lanes : Anti-SUN2 antibody [EPR6556] (ab133591) at 1/1000 dilution
Lane 1 : Human fetal muscle tissue lysate
Lane 2 : Saos 2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 80 kDa
ab133591 at 1/30 dilution immunoprecipitating SUN2 in Saos-2 (human osteosarcoma) cell lysate. Total sample is 300µg. The large spread out band at 50 kDa is Rabbit IgG heavy chain.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133591 has been referenced in 1 publication.
- Sun WW et al. SUN2 Modulates HIV-1 Infection and Latency through Association with Lamin A/C To Maintain the Repressive Chromatin. MBio 9:N/A (2018). PubMed: 29717016