Recombinant Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
![Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)](https://www.abcam.com/ps/products/248/ab248569/Images/ab248569-405975-anti-sun2-antibody-epr6556-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6556] to SUN2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-SUN2 antibody [EPR6556] - BSA and Azide free
See all SUN2 primary antibodies -
Description
Rabbit monoclonal [EPR6556] to SUN2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248569 is the carrier-free version of ab133591 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248569 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.01 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6556 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa. |
Target
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Relevance
SUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope. -
Cellular localization
Nuclear membrane, endosome membrane, mitotic spindle organization. -
Database links
- Entrez Gene: 25777 Human
- Omim: 613569 Human
- SwissProt: Q9UH99 Human
- Unigene: 517622 Human
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Alternative names
- FRIGG antibody
- KIAA0668 antibody
- nuclear envelope protein antibody
see all
Images
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Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)All lanes : Anti-SUN2 antibody [EPR6556] (ab133591) at 1/1000 dilution
Lane 1 : Human fetal muscle tissue lysate
Lane 2 : Saos 2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 80 kDaThis data was developed using ab133591, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)](https://www.abcam.com/ps/products/248/ab248569/Images/ab248569-2-anti-sun2-antibody-epr6556-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569) -
![OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)](https://www.abcam.com/ps/products/248/ab248569/Images/ab248569-1-anti-sun2-antibody-epr6556-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)This data was developed using ab133591, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)](https://www.abcam.com/ps/products/248/ab248569/Images/ab248569-4-benefits-of-recombinant-antibodies.png)
Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab248569 has not yet been referenced specifically in any publications.
