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RecombinantRabMAb

Recombinant Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
  • Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
  • OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
  • Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6556] to SUN2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human SUN2 protein (ab132707)

View more associated products

Overview

  • Product name

    Anti-SUN2 antibody [EPR6556] - BSA and Azide free
    See all SUN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6556] to SUN2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248569 is the carrier-free version of ab133591 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248569 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.01 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6556
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export

Associated products

  • Alternative Versions

    • Anti-SUN2 antibody [EPR6556] (ab133591)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human SUN2 protein (ab132707)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Relevance

      SUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
    • Cellular localization

      Nuclear membrane, endosome membrane, mitotic spindle organization.
    • Database links

      • Entrez Gene: 25777 Human
      • Omim: 613569 Human
      • SwissProt: Q9UH99 Human
      • Unigene: 517622 Human
      • Alternative names

        • FRIGG antibody
        • KIAA0668 antibody
        • nuclear envelope protein antibody
        • Protein unc-84 homolog B antibody
        • Rab5 interacting protein antibody
        • RAB5IP antibody
        • Sad1 and UNC84 domain containing 2 antibody
        • Sad1 unc-84 domain protein 2 antibody
        • Sad1 unc84 domain protein antibody
        • Sad1/unc-84 protein-like 2 antibody
        • Sad1/unc84 protein-like antibody
        • SUN domain-containing protein 2 antibody
        • UNC 84B antibody
        • unc84 homolog B (C. elegans) antibody
        • unc84 homolog B antibody
        • unc84, C. elegans, homolog of, B antibody
        • UNC84B antibody
        see all

      Images

      • Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        All lanes : Anti-SUN2 antibody [EPR6556] (ab133591) at 1/1000 dilution

        Lane 1 : Human fetal muscle tissue lysate
        Lane 2 : Saos 2 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : HepG cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 80 kDa



        This data was developed using ab133591, the same antibody clone in a different buffer formulation.

      • Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        This data was developed using ab133591, the same antibody clone in a different buffer formulation.ab133591 at 1/30 dilution immunoprecipitating SUN2 in Saos-2 (human osteosarcoma) cell lysate. Total sample is 300µg. The large spread out band at 50 kDa is Rabbit IgG heavy chain.
      • OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        This data was developed using ab133591, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)
        Anti-SUN2 antibody [EPR6556] - BSA and Azide free (ab248569)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab248569? Please let us know so that we can cite the reference in this datasheet.

      ab248569 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab248569.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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