Recombinant
RabMAb

Recombinant Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

Overview

  • Product name

    Anti-SUN2 antibody [EPR6557] - BSA and Azide free
    See all SUN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6557] to SUN2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SUN2 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9UH99

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab232365 is the carrier-free version of ab124916. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232365 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    SUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
  • Cellular localization

    Nuclear membrane, endosome membrane, mitotic spindle organization.
  • Database links

  • Alternative names

    • FRIGG antibody
    • KIAA0668 antibody
    • nuclear envelope protein antibody
    • Protein unc-84 homolog B antibody
    • Rab5 interacting protein antibody
    • RAB5IP antibody
    • Sad1 and UNC84 domain containing 2 antibody
    • Sad1 unc-84 domain protein 2 antibody
    • Sad1 unc84 domain protein antibody
    • Sad1/unc-84 protein-like 2 antibody
    • Sad1/unc84 protein-like antibody
    • SUN domain-containing protein 2 antibody
    • UNC 84B antibody
    • unc84 homolog B (C. elegans) antibody
    • unc84 homolog B antibody
    • unc84, C. elegans, homolog of, B antibody
    • UNC84B antibody
    see all

Images

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SUN2 (red) with ab124916 at a 1/30 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • ab124916 staining SUN2 in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated goat anti-rabbit IgG, ab97051 (1/500) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human lung tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human ovarian tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • ab124916 staining SUN2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An  HRP-conjugated Goat anti-rabbit IgG, ab97051 (1/500), was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

References

ab232365 has not yet been referenced specifically in any publications.

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