Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)
Key features and details
- Mouse monoclonal [2F5] to Superoxide Dismutase 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Superoxide Dismutase 1 antibody [2F5]
See all Superoxide Dismutase 1 primary antibodies -
Description
Mouse monoclonal [2F5] to Superoxide Dismutase 1 -
Host species
Mouse -
Specificity
ab115750 does not cross react with immunoglobulins but does react weakly with Mn SOD -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Tissue, cells or virus corresponding to Cow Superoxide Dismutase 1. Superoxide dismutase enzyme prepared from bovine erythrocytes
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Positive control
- Human Kidney and Human Liver tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2F5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Post-translational
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
Cellular localization
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 281495 Cow
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00442 Cow
- SwissProt: P00441 Human
- Unigene: 443914 Human
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Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
Images
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Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115750 at 15 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Human Liver (formalin-fixed, paraffin-embedded) stained with ab115750 at 15 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab115750 has not yet been referenced specifically in any publications.