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    superoxide-dismutase-1-antibody-ab13498.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Superoxide Dismutase 1 antibody (ab13498)

  • Datasheet
  • SDS
Reviews (23)Q&A (9)References (99)

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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)

    Key features and details

    • Rabbit polyclonal to Superoxide Dismutase 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Superoxide Dismutase 1 antibody
      See all Superoxide Dismutase 1 primary antibodies
    • Description

      Rabbit polyclonal to Superoxide Dismutase 1
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Full length protein corresponding to Human Superoxide Dismutase 1.
      Database link: P00441

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituents: PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was purified on an antigen coupled sepharose column.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins
      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Antioxidants
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Assay kits

      • Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Superoxide Dismutase 1 protein (Active) (ab112193)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab13498 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (13)
    Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001.
    Notes
    WB
    Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001.

    Target

    • Function

      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in disease

      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • Sequence similarities

      Belongs to the Cu-Zn superoxide dismutase family.
    • Post-translational
      modifications

      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • Cellular localization

      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Target information above from: UniProt accession P00441 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6647 Human
      • Omim: 147450 Human
      • SwissProt: P00441 Human
      • Unigene: 443914 Human
      • Alternative names

        • ALS antibody
        • ALS1 antibody
        • Amyotrophic lateral sclerosis 1 adult antibody
        • Cu/Zn SOD antibody
        • Cu/Zn superoxide dismutase antibody
        • Epididymis secretory protein Li 44 antibody
        • HEL S 44 antibody
        • Homodimer antibody
        • hSod1 antibody
        • Indophenoloxidase A antibody
        • IPOA antibody
        • Mn superoxide dismutase antibody
        • SOD antibody
        • SOD soluble antibody
        • SOD1 antibody
        • SOD2 antibody
        • SODC antibody
        • SODC_HUMAN antibody
        • Superoxide dismutase [Cu-Zn] antibody
        • Superoxide dismutase 1 antibody
        • Superoxide dismutase 1 soluble antibody
        • Superoxide dismutase Cu Zn antibody
        • Superoxide dismutase cystolic antibody
        see all

      Images

      • Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)
        Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)
        Anti-Superoxide Dismutase 1 antibody (ab13498) at 0.2 µg/ml (2 hrs at RT) + HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 15 µg

        Secondary
        Donkey Anti-Rabbit IgG HRP 1 hr at RT

        Predicted band size: 18 kDa



        Block: 1.5% BSA.

      Protocols

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (99)

      Publishing research using ab13498? Please let us know so that we can cite the reference in this datasheet.

      ab13498 has been referenced in 99 publications.

      • Hu W  et al. Green Tea Polyphenols Modulated Cerebral SOD Expression and Endoplasmic Reticulum Stress in Cardiac Arrest/Cardiopulmonary Resuscitation Rats. Biomed Res Int 2020:5080832 (2020). PubMed: 32185207
      • Feng M  et al. H19/miR-130a-3p/DAPK1 axis regulates the pathophysiology of neonatal hypoxic-ischemia encephalopathy. Neurosci Res N/A:N/A (2020). PubMed: 32173462
      • Xu W  et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
      • Seminary ER  et al. Motor Neuron Generation from iPSCs from Identical Twins Discordant for Amyotrophic Lateral Sclerosis. Cells 9:N/A (2020). PubMed: 32121108
      • Wang J  et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med 24:3931-3947 (2020). PubMed: 32126150
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 32 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Superoxide Dismutase 1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (SH-SY5Y cell line)
      Permeabilization
      Yes - 0.05% Triton X-100 in PBS 1X
      Specification
      SH-SY5Y cell line
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 12 2020

      Western blot abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (SH-SY5Y cell line)
      Gel Running Conditions
      Reduced Denaturing (15%)
      Loading amount
      20 µg
      Specification
      SH-SY5Y cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 12 2020

      Immunocytochemistry abreview for Anti-Superoxide Dismutase 1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Rat Cultured Cells (Cardiomyocytes)
      Permeabilization
      Yes - Triton x-100, 0.03%
      Specification
      Cardiomyocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 28 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (retina)
      Permeabilization
      No
      Specification
      retina
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 07 2017

      Western blot abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (retina)
      Gel Running Conditions
      Non-reduced Denaturing (10% TGX gel)
      Loading amount
      30 µg
      Specification
      retina
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 07 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain 20um thick)
      Permeabilization
      Yes - 0.1% triton-x
      Specification
      Brain 20um thick
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Kelly Maers

      Verified customer

      Submitted Jan 24 2017

      Western blot abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Silk worm Tissue lysate - whole (testis lysate (silkworm), BmN4 cell lysate (silkwo)
      Gel Running Conditions
      Non-reduced Denaturing (12% polyacrylamide gel)
      Loading amount
      10 µg
      Specification
      testis lysate (silkworm), BmN4 cell lysate (silkwo
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      陽水 野島

      Verified customer

      Submitted Aug 17 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Cow Cell (aorta endothelial cells)
      Permeabilization
      Yes - Saponin 0,1%
      Specification
      aorta endothelial cells
      Blocking step
      PBS/Saponin0,1%/BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Fixative
      PFA 4%
      Read More

      Miss. Virginie Joris

      Verified customer

      Submitted Jun 30 2015

      Western blot abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Skeletal muscle)
      Gel Running Conditions
      Reduced Denaturing (15% Gel)
      Loading amount
      20 µg
      Specification
      Skeletal muscle
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 19 2015

      Western blot abreview for Anti-Superoxide Dismutase 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Skeletal muscle)
      Gel Running Conditions
      Reduced Denaturing (15% Gel)
      Loading amount
      20 µg
      Specification
      Skeletal muscle
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 19 2015

      1-10 of 32 Abreviews or Q&A

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