Anti-Superoxide Dismutase 1 antibody (ab13498)
- Datasheet
- Specific References (52)
- Protocols
Overview
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Product nameAnti-Superoxide Dismutase 1 antibody
See all Superoxide Dismutase 1 primary antibodies -
DescriptionRabbit polyclonal to Superoxide Dismutase 1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IP, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey, African green monkey
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Immunogen
Full length protein corresponding to Human Superoxide Dismutase 1.
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Positive control
- IHC-P: Human placenta tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was purified on an antigen coupled sepharose column.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Corresponding Antibody
Applications
Our Abpromise guarantee covers the use of ab13498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000. See Abreview. | |
| IHC-Fr | 1/1000. | |
| WB | Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001. | |
| IP | Use a concentration of 10 µg/ml. See Shinder et al 2001. | |
| ELISA | Use at an assay dependent concentration. See Downs et al 2002. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
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Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
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Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
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Post-translational
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
- Information by UniProt
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Database links
- Entrez Gene: 281495 Cow
- Entrez Gene: 39251 Drosophila melanogaster
- Entrez Gene: 6647 Human
- Entrez Gene: 20655 Mouse
- Entrez Gene: 397036 Pig
- Entrez Gene: 100009313 Rabbit
- Entrez Gene: 24786 Rat
- Entrez Gene: 100270717 Sheep
see all -
Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
Images
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an anonymous Abreview.Anti-Superoxide Dismutase 1 antibody (ab13498) at 1/5000 dilution + Whole rat tissue lysate at 20 µg
Secondary
HRP-conjugated donkey anti-rabbit polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Exposure time: 60 secondsBlocking: 5% BSA for 1 hour at 20o.
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an anonymous Abreview.All lanes : Anti-IL18 antibody (ab71495) at 1/1000 dilution
All lanes : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 15 kDa (why is the actual band size different from the predicted?)
Exposure time: 140 secondsBlocked with 3% milk for 2 hours at 25°C.
Incubated with the primary antibody for 16 hours at 4°C in 3% milk in TBS-Tween (0.005%).
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Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an Abreview submitted by Virginie Joris.ab71495 staining Superoxide Dismutase 1 in cow aorta endothelial cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% saponin and blocked with PBS + 0.1% saponin + 1% BSA for 1 hour at 24°C. Samples were incubated with the primary antibody (1/200 in PBS + 0.1% saponin + 1% BSA) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody at a dilution of 1/300.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab13498)ab13498 at 1µg/ml staining Superoxide dismutase 1 in human placenta tissue section by Immunohistochemistry ((Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti-rabbit secondary was used at 1/10 dilution.
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Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 1 antibody (ab13498)Image courtesy of an anonymous Abreview.ab13498 staining Superoxide Dismutase 1 in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an anonymous Abreview.ab13498 (1/200) staining Human cell line 293FT by ICC/IF. The 293 FT cells were cultured for 3 days, fixed in 3.7% formaldehyde and blocked with 5% BSA in PBS for 1 hr. The secondary antibody was goat anti-Rabbit IgG conjugated to Alexa Fluor® 488 (green) and the nucleus (blue) were stained with DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an Anonymous Abreview.ab13498 staining Superoxide Dismutase 1 in mouse bone marrow white blood cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 12 hours at 4°C. The secondary antibody used was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (polyclonal), diluted to 1/500.
Nuclei were counterstained blue with DAPI.
Protocols
References
This product has been referenced in:
- Szelechowski M et al. Metabolic Reprogramming in Amyotrophic Lateral Sclerosis. Sci Rep 8:3953 (2018). Read more (PubMed: 29500423) »
- Seminary ER et al. Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons. Front Neurosci 12:86 (2018). Read more (PubMed: 29515358) »
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