Overview

  • Product name
    Anti-Superoxide Dismutase 1 antibody
    See all Superoxide Dismutase 1 primary antibodies
  • Description
    Rabbit polyclonal to Superoxide Dismutase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Full length protein (Rat).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa).
IP Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similarities
    Belongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    modifications
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localization
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Images

  • Anti-Superoxide Dismutase 1 antibody (ab13499) at 1 µg/ml + Cell lysates prepared from mixed human cell lines (A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G)

    Predicted band size: 18 kDa

  • Superoxide Dismutase 1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Superoxide Dismutase 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13499.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 19kDa; Superoxide Dismutase 1

  • Ab13499 staining Human normal colon. Staining is localized to the nucleus.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Buglak NE  et al. Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats. Redox Biol 19:166-178 (2018). Read more (PubMed: 30172101) »
  • Gasperini L  et al. In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. Front Neurosci 10:437 (2016). Read more (PubMed: 27729845) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

Dear Supplier,

Please, could you send me the datasheet about the following item:

https://www.abcam.com/Superoxide-Dismutase-1-antibody-ab13499.html

Is the product in stock? Is there some discount for us?

Thank you in advance!

If you have any questions, please do not hesitate to contact me.

Kind regards,

Iva Pavlina

http://www.gentaur.com/

http://antibody-antibodies.com/











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Contact me with SKYPE: iva_pavlina_bulgaria

Read More
Answer

Thank you for your enquiry.

I can confirm that the datasheet for this product is the link you have sent within your email. I have also attached a PDF copy of this and another link below:

ab13499
https://www.abcam.com/index.html?datasheet=13499 (or use the following: https://www.abcam.com/index.html?datasheet=13499).

The online datasheet is up to date and will let you know if there is stock,itwill alsolet you know when it will be delivered and if there is any delay. At the moment I can confirmwe do have stock available of ab13499 Superoxide Dismutase 1 antibody.

I am sorry there are currently no discounts available for this particular product. We do offer bulk discounts for orders of 5 items or more.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry. The difference between the products is the immunogen sequence, one is rat and the other is human. A mistake had occurred in the publishing of this data. Thank you for letting us know about this mistake on our enquiries page. Our goal here at Abcam is to answer customer enquiries as accurately, honestly and as quickly as possible. From time to time, our technical team will make mistakes - when this happens we rely on researchers to let us know. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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