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  1. Link

    superoxide-dismutase-1-antibody-ab183881.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Superoxide Dismutase 1 antibody (ab183881)

  • Datasheet
  • SDS
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  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
  • Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab183881)
  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)
  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody
    See all Superoxide Dismutase 1 primary antibodies
  • Description

    Rabbit polyclonal to Superoxide Dismutase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment within Human Superoxide Dismutase 1 aa 75-154. The exact sequence is proprietary.
    Database link: P00441

  • Positive control

    • NT2D1 , PC-3 , U87-MG , SK-N-SH , HeLa , Rat brain lysates, Hela cells, colon carcinoma, muscle, rat brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • PC3 whole cell lysate (ab3954)
    • SK N SH whole cell lysate (ab3956)
  • Recombinant Protein

    • Recombinant human Superoxide Dismutase 1 protein (Active) (ab112193)
  • Related Products

    • HeLa whole cell lysate (ab150035)
    • Recombinant Human Superoxide Dismutase 1 protein (ab153789)
    • HeLa whole cell lysate (ab29545)
    • Recombinant human Superoxide Dismutase 1 protein (ab74916)
    • Recombinant Human Superoxide Dismutase 1 protein (ab84642)

Applications

Our Abpromise guarantee covers the use of ab183881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease

    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similarities

    Belongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    modifications

    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localization

    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Target information above from: UniProt accession P00441 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 449637 Chimpanzee
    • Entrez Gene: 281495 Cow
    • Entrez Gene: 6647 Human
    • Entrez Gene: 20655 Mouse
    • Entrez Gene: 397036 Pig
    • Entrez Gene: 24786 Rat
    • Entrez Gene: 574096 Rhesus monkey
    • Entrez Gene: 100270717 Sheep
    • Entrez Gene: 549006 Xenopus tropicalis
    • Entrez Gene: 30553 Zebrafish
    • Omim: 147450 Human
    • SwissProt: P60052 Chimpanzee
    • SwissProt: P00442 Cow
    • SwissProt: P00441 Human
    • SwissProt: P08228 Mouse
    • SwissProt: P04178 Pig
    • SwissProt: P07632 Rat
    • SwissProt: Q8HXQ0 Rhesus monkey
    • SwissProt: P09670 Sheep
    • SwissProt: O73872 Zebrafish
    • Unigene: 443914 Human
    • Unigene: 276325 Mouse
    • Unigene: 466779 Mouse
    • Unigene: 6059 Rat
    • Unigene: 75822 Zebrafish
    see all
  • Alternative names

    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Images

  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
    All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC-3 whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 16 kDa



    15% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab183881)

    Immunocytochemical analysis of methanol fixed Hela cells labeling Superoxide Dismutase 1 at 1/100 dilution with ab183881.  Bottom panel shows co-staining with Hoechst 33342 (blue)

  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 16 kDa



    15% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)

    Immunohistochemical analysis of paraffin-embedded rat brain labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)

    Immunohistochemical analysis of paraffin-embedded mouse Muscle labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)

    Immunohistochemical staining of paraffin-embedded human colon carcinoma labeling Superoxide Dismutase 1 at 1/500 dilution with ab183881

  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
    Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)
    All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution

    Lane 1 : Hela mock control
    Lane 2 : HeLa transfected shSuperoxide Dismutase 1

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 16 kDa



    15% SDS-PAGE

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Maugeri G  et al. Molecular mechanisms involved in the protective effect of pituitary adenylate cyclase-activating polypeptide in an in vitro model of amyotrophic lateral sclerosis. J Cell Physiol 234:5203-5214 (2019). Read more (PubMed: 30238989) »
    See 1 Publication for this product

    Publishing research using ab183881? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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