- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Superoxide Dismutase 1 antibody
See all Superoxide Dismutase 1 primary antibodies -
DescriptionRabbit polyclonal to Superoxide Dismutase 1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Xenopus tropicalis
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Immunogen
Recombinant fragment within Human Superoxide Dismutase 1 aa 75-154. The exact sequence is proprietary.
Database link: P00441 -
Positive control
- NT2D1 , PC-3 , U87-MG , SK-N-SH , HeLa , Rat brain lysates, Hela cells, colon carcinoma, muscle, rat brain tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
- HeLa whole cell lysate (ab150035)
- Recombinant Human Superoxide Dismutase 1 protein (ab153789)
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- HeLa whole cell lysate (ab29545)
- Brain (rat) normal tissue slides (ab4616)
- Recombinant human Superoxide Dismutase 1 protein (ab73795)
- Recombinant human Superoxide Dismutase 1 protein (ab74916)
- Recombinant Human Superoxide Dismutase 1 protein (ab84642)
- Recombinant human Superoxide Dismutase 1 protein (ab90040)
Applications
Our Abpromise guarantee covers the use of ab183881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 16 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. |
Target
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FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
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Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
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Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
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Post-translational
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
- Information by UniProt
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Database links
- Entrez Gene: 449637 Chimpanzee
- Entrez Gene: 281495 Cow
- Entrez Gene: 6647 Human
- Entrez Gene: 20655 Mouse
- Entrez Gene: 397036 Pig
- Entrez Gene: 24786 Rat
- Entrez Gene: 574096 Rhesus monkey
- Entrez Gene: 100270717 Sheep
see all -
Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
Images
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : PC-3 whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : SK-N-SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa15% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab183881)Immunocytochemical analysis of methanol fixed Hela cells labeling Superoxide Dismutase 1 at 1/100 dilution with ab183881. Bottom panel shows co-staining with Hoechst 33342 (blue)
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 16 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)Immunohistochemical analysis of paraffin-embedded rat brain labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)Immunohistochemical analysis of paraffin-embedded Muscle labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab183881)Immunohistochemical staining of paraffin-embedded colon carcinoma labeling Superoxide Dismutase 1 at 1/500 dilution with ab183881
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881)All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution
Lane 1 : Hela mock control
Lane 2 : HeLa transfected shSuperoxide Dismutase 1
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa15% SDS-PAGE
Protocols
References
ab183881 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"