Key features and details
- Rabbit polyclonal to Superoxide Dismutase 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Superoxide Dismutase 1 antibody
See all Superoxide Dismutase 1 primary antibodies
DescriptionRabbit polyclonal to Superoxide Dismutase 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken
Recombinant full length protein (His-T7-tag) corresponding to Chicken Superoxide Dismutase 1 aa 2-154. Expressed in E. coli. N-terminal tags.
ATLKAVCVMKGDAPVEGVIHFQQQGSGPVKVTGKITGLSDGDHGFHVHEF GDNTNGCTSAGAHFNPEGKQHGGPKDADRHVGDLGNVTAKGGVAEVEIED SVISLTGPHCIIGRTMVVHAKSDDLGRGGDNESKLTGNAGPRLACGVIGI AKC
Database link: P80566
- WB: Recombinant chicken Superoxide Dismutase 1 protein. Jurkat and HeLa cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
- Information by UniProt
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
Anti-Superoxide Dismutase 1 antibody (ab232863) at 3 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Anti-Superoxide Dismutase 1 antibody (ab232863) at 3 µg/ml + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Anti-Superoxide Dismutase 1 antibody (ab232863) at 2 µg/ml + Recombinant chicken Superoxide Dismutase 1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232863 has not yet been referenced specifically in any publications.