Key features and details
- Rabbit polyclonal to Superoxide Dismutase 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Superoxide Dismutase 1 antibody
See all Superoxide Dismutase 1 primary antibodies
DescriptionRabbit polyclonal to Superoxide Dismutase 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Full length native protein (purified) corresponding to Cow Superoxide Dismutase 1. Superoxide dismutase from bovine erythrocytes.
- WB: Human, mouse and rat liver lysate. IHC-P: Human liver tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab256348 in the following tested applications.
|WB||Use a concentration of 0.025 - 0.1 µg/ml. Predicted molecular weight: 16 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
- Information by UniProt
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
All lanes : Anti-Superoxide Dismutase 1 antibody (ab256348) at 0.025 µg/ml
Lane 1 : Human liver lysate
Lane 2 : Mouse liver lysate
Lane 3 : Rat liver lysate
Predicted band size: 16 kDa
Paraffin-embedded human liver tissue stained for Superoxide Dismutase 1 using ab256348 at 5μg/ml in immunohistochemical analysis (bottom panel) as compared to an isotype control (top panel).
ab256348 has not yet been referenced specifically in any publications.