Overview

  • Product name
    Anti-Superoxide Dismutase 1 antibody
    See all Superoxide Dismutase 1 primary antibodies
  • Description
    Rabbit polyclonal to Superoxide Dismutase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human Superoxide Dismutase 1

  • Positive control
    • Jurkat cell lysate, human placenta and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab52950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/20.
IHC-P 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in disease
      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • Sequence similarities
      Belongs to the Cu-Zn superoxide dismutase family.
    • Post-translational
      modifications
      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • Cellular localization
      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    Images

    • Anti-Superoxide Dismutase 1 antibody (ab52950) at 1/5000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 16 kDa

    • ab52950 at 1/50 dilution staining Superoxide Dismutase 1 in human placenta by Immunohistochemistry, Paraffin embedded tissue.

    References

    This product has been referenced in:
    • Shvil N  et al. MIF inhibits the formation and toxicity of misfolded SOD1 amyloid aggregates: implications for familial ALS. Cell Death Dis 9:107 (2018). WB . Read more (PubMed: 29371591) »
    • Wang TH  et al. Fisetin Exerts Antioxidant and Neuroprotective Effects in Multiple Mutant hSOD1 Models of Amyotrophic Lateral Sclerosis by Activating ERK. Neuroscience 379:152-166 (2018). IF . Read more (PubMed: 29559385) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Bos taurus Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Julie Kim

    Verified customer

    Submitted May 12 2017

    Answer


    Our cross reactivity recommendations for 1926-1 and 2582-1 are based off of Western blot analysis only. We do not recommend either product for detection of mouse SOD1 as we did not obtain any staining in WB with 2582-1, and we obtained non-specific staining only with 1926-1. There is 78% homology between the immunogen sequence (which is the same for both products) and mouse SOD1. Based on these results, we do not predict that either antibody would work in ICC/IF for mouse samples.
    I've attached the western blot image for 2582-1 in mouse.

    Read More

    Answer

    Vielen Dank für Ihre Antwort.

    Ich kann bestätigen, dass die Immunogensequenz von ab52950die Mutation G93A nicht beinhaltet und somit beide Proteine erkennen wird. (Ich nehme hierbei an, dass G93A eine Punktmutation ist, die nicht zu einem trunkierten Protein führt).

    ab16831 ist ein polyklonaler Antikörper, dessen Immunogen das Protein voller Länge war, und es ist somit höchst wahrscheinlich, dass auch er beide Formen, mutiert und WT, erkennt. Dies ist aber nicht experimentell von uns getestet worden.

    I hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Masterarbeit.

    Read More

    Answer

    Vielen Dank für Ihre Anfrage.
    Ich freue mich Ihnen mitteilen zu können, dass beide Lots eine Konzentration von 0.263 mg/mL haben.
    Ich möchte auch darauf hinweisen, dass diese scheinbar niedrige Konzentration völlig ausreichend ist. Dieser Antikörper ist ein monoklonaler Antikörper aus dem Kaninchen. Kaninchen produzieren extrem spezifische Antikörper mit einer sehr starken Affinität und somit wird weniger Antikörper benötigt als mit monoklonalen aus der Maus. Die Menge an "Tests", die mit einem Röhrchen durchgeführt werden kann, wird von uns streng kontrolliert und immer vergleichbar gehalten.
    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
    Loading amount
    15 µg
    Specification
    1 EBNA, 2 HeLa
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%

    Abcam user community

    Verified customer

    Submitted Dec 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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