Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody
    See all Superoxide Dismutase 1 primary antibodies
  • Description

    Goat polyclonal to Superoxide Dismutase 1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Deer, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Superoxide Dismutase 1 aa 69-81 (internal sequence).
    Sequence:

    C-SRKHGGPKDEERH

  • Positive control

    • Recombinant human Superoxide Dismutase 1 protein (ab74916) can be used as a positive control in WB. WB: Mouse brain tissue lysate and NIH3T3, HEK293, HepG2 and MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease

    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similarities

    Belongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    modifications

    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localization

    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Images

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab62800) at 0.01 µg/ml

    Lane 1 : NIH3T3 cell lysate in RIPA buffer
    Lane 2 : HEK293 cell lysate in RIPA buffer
    Lane 3 : HepG2 cell lysate in RIPA buffer
    Lane 4 : MCF7 cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 16 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • ab62800 (1/200) staining Superoxide Dismutase 1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus. Please refer to Abreview for further experimental details.

    See Abreview

  • Anti-Superoxide Dismutase 1 antibody (ab62800) at 0.02 µg/ml + Mouse brain tissue lysate in RIPA buffer at 35 µg

    Predicted band size: 16 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:

  • Juttukonda LJ  et al. Dietary Manganese Promotes Staphylococcal Infection of the Heart. Cell Host Microbe 22:531-542.e8 (2017). Read more (PubMed: 28943329) »
  • Ganapathy E  et al. D-4F, an apoA-I mimetic peptide, inhibits proliferation and tumorigenicity of epithelial ovarian cancer cells by upregulating the antioxidant enzyme MnSOD. Int J Cancer 130:1071-81 (2012). WB ; Mouse . Read more (PubMed: 21425255) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 12 2009

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