RecombinantRabMAb

Recombinant Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody [EP1727Y]
    See all Superoxide Dismutase 1 primary antibodies

  • Description

    Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Mouse cerebrum, rat liver and human breast carcinoma tissue WB: Jurkat cell lysate; Mouse and rat brain tissue lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab51254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

    • Involvement in disease

      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.

    • Sequence similarities

      Belongs to the Cu-Zn superoxide dismutase family.

    • Post-translational
      modifications

      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.

    • Cellular localization

      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    Images

    • Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      All lanes : Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 0.015 µg/ml

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

      Predicted band size: 16 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?



      Blocking/diluting Buffer and concentration: 5% NFDM /TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
    • Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Superoxide Dismutase 1 with ab51254 at 1/20 dilution (1 µg) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).

       

    • Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunofluorescent staining of HeLa cells using ab51254 (1:100).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

      Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
      Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (10)

    Publishing research using ab51254? Please let us know so that we can cite the reference in this datasheet.

    ab51254 has been referenced in 10 publications.

    • Li X  et al. The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network. Oxid Med Cell Longev 2019:9706792 (2019). PubMed: 30911355
    • Chai W  et al. HMGB1-mediated autophagy regulates sodium/iodide symporter protein degradation in thyroid cancer cells. J Exp Clin Cancer Res 38:325 (2019). PubMed: 31331356
    • Duan W  et al. Deletion of Tbk1 disrupts autophagy and reproduces behavioral and locomotor symptoms of FTD-ALS in mice. Aging (Albany NY) 11:2457-2476 (2019). PubMed: 31039129
    • Auber M  et al. Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles. J Extracell Vesicles 8:1656042 (2019). PubMed: 31552133
    • Soares MB  et al. The Step of Incorporation of Bacillus coagulans GBI-30 6086 Into "requeijão cremoso" Processed Cheese Does Not Affect Metabolic Homeostasis of Rats. Front Microbiol 10:2332 (2019). PubMed: 31695686
    • Duan X  et al. Autophagy inhibition attenuates hyperoxaluria-induced renal tubular oxidative injury and calcium oxalate crystal depositions in the rat kidney. Redox Biol 16:414-425 (2018). PubMed: 29653411
    • Gong ZG  et al. Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem 192:62-71 (2018). PubMed: 30599315
    • Liu Q  et al. Proteomic profiling for plasma biomarkers of tuberculosis progression. Mol Med Rep 18:1551-1559 (2018). PubMed: 29901122
    • Liu KM  et al. Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum. Am J Physiol Renal Physiol 309:F318-31 (2015). IF ; Rat . PubMed: 26109091
    • Moura CS  et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . PubMed: 24465380

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody [EP1727Y]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Ovary)
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Ms. Natasja Costermans

    Verified customer

    Submitted Aug 07 2018

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