Recombinant
Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Superoxide Dismutase 1 antibody [EP1727Y]
See all Superoxide Dismutase 1 primary antibodies -
DescriptionRabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.
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Positive control
- Jurkat cell lysate, human breast carcinoma tissue or HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1727Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). | |
| Flow Cyt | 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/250. |
Application notes
Is unsuitable for IP.
Target
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FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
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Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
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Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
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Post-translational
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
- Information by UniProt
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Database links
- Entrez Gene: 6647 Human
- Entrez Gene: 24786 Rat
- Omim: 147450 Human
- SwissProt: P00441 Human
- SwissProt: P07632 Rat
- Unigene: 443914 Human
- Unigene: 6059 Rat
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Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
Images
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![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)](https://www.abcam.com/ps/products/51/ab51254/Images/ab51254_1.jpg)
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution + Jurkat cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)](https://www.abcam.com/ps/products/51/ab51254/Images/ab51254_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue. -
![Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)](https://www.abcam.com/ps/products/51/ab51254/Images/ab51254_3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)Immunofluorescent staining of HeLa cells using ab51254 (1:100).
Datasheets and documents
References
This product has been referenced in:
- Duan X et al. Autophagy inhibition attenuates hyperoxaluria-induced renal tubular oxidative injury and calcium oxalate crystal depositions in the rat kidney. Redox Biol 16:414-425 (2018). Read more (PubMed: 29653411) »
- Gong ZG et al. Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem 192:62-71 (2018). Read more (PubMed: 30599315) »
