Recombinant
RabMAb

Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Abreviews (1) Submit a Question Specific References (2)

Overview

  • Product name
    Anti-Superoxide Dismutase 1 antibody [EP1727Y]
    See all Superoxide Dismutase 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • Jurkat cell lysate, human breast carcinoma tissue or HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in disease
      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • Sequence similarities
      Belongs to the Cu-Zn superoxide dismutase family.
    • Post-translational
      modifications
      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • Cellular localization
      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt
    • Database links
      • Alternative names
        • ALS antibody
        • ALS1 antibody
        • Amyotrophic lateral sclerosis 1 adult antibody
        • Cu/Zn SOD antibody
        • Cu/Zn superoxide dismutase antibody
        • Epididymis secretory protein Li 44 antibody
        • HEL S 44 antibody
        • Homodimer antibody
        • hSod1 antibody
        • Indophenoloxidase A antibody
        • IPOA antibody
        • Mn superoxide dismutase antibody
        • SOD antibody
        • SOD soluble antibody
        • SOD1 antibody
        • SOD2 antibody
        • SODC antibody
        • SODC_HUMAN antibody
        • Superoxide dismutase [Cu-Zn] antibody
        • Superoxide dismutase 1 antibody
        • Superoxide dismutase 1 soluble antibody
        • Superoxide dismutase Cu Zn antibody
        • Superoxide dismutase cystolic antibody
        see all

      Images

      • Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
        Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
        Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution + Jurkat cell lysate at 10 µg

        Secondary
        Goat anti-rabbit HRP labeled at 1/2000 dilution

        Predicted band size: 16 kDa
        Observed band size: 18 kDa (why is the actual band size different from the predicted?)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
        Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
      • Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
        Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
        Immunofluorescent staining of HeLa cells using ab51254 (1:100).

      References

      This product has been referenced in:
      • Liu KM  et al. Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum. Am J Physiol Renal Physiol 309:F318-31 (2015). IF ; Rat . Read more (PubMed: 26109091) »
      • Moura CS  et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . Read more (PubMed: 24465380) »

      See all 2 Publications for this product

      Publishing research using ab51254? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1 Review or Q&A

      10
      Votes: Highest First

      Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody [EP1727Y]

       Good
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Pig Tissue sections (Ovary)
      Permeabilization
      No
      Specification
      Ovary
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      abreview image
      Username

      Natasja Costermans

      Verified customer

      Submitted Aug 07 2018

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