Overview

  • Product name
    Anti-Superoxide Dismutase 1 antibody [EP1727Y]
    See all Superoxide Dismutase 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • Jurkat cell lysate, human breast carcinoma tissue or HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in disease
      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • Sequence similarities
      Belongs to the Cu-Zn superoxide dismutase family.
    • Post-translational
      modifications
      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • Cellular localization
      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    Images

    • Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?

    • Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab51254 (1:100).

    References

    This product has been referenced in:
    • Duan X  et al. Autophagy inhibition attenuates hyperoxaluria-induced renal tubular oxidative injury and calcium oxalate crystal depositions in the rat kidney. Redox Biol 16:414-425 (2018). Read more (PubMed: 29653411) »
    • Liu KM  et al. Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum. Am J Physiol Renal Physiol 309:F318-31 (2015). IF ; Rat . Read more (PubMed: 26109091) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Ovary)
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Ms. Natasja Costermans

    Verified customer

    Submitted Aug 07 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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