Recombinant
RabMAb

Recombinant Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free
    See all Superoxide Dismutase 1 primary antibodies
  • Description

    Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Ab238954 is the carrier-free version of ab51254. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238954 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in disease

      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • Sequence similarities

      Belongs to the Cu-Zn superoxide dismutase family.
    • Post-translational
      modifications

      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • Cellular localization

      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    Images

    • Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254).

    • Immunofluorescent staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab51254 (1:100).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254).

    References

    ab238954 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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