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Hello, I am looking for an antibody specific of the human SOD1, which does not recognize the mouse SOD1.
Have you got any data that indicates that the antibodies Cat# 1926-1 or 2582-1 could be OK for me in immunofluorescence? As the data sheet mentions a specificity for the human form only in Western Blot.
Asked on May 02 2013
Our cross reactivity recommendations for 1926-1 and 2582-1 are based off of Western blot analysis only. We do not recommend either product for detection of mouse SOD1 as we did not obtain any staining in WB with 2582-1, and we obtained non-specific staining only with 1926-1. There is 78% homology between the immunogen sequence (which is the same for both products) and mouse SOD1. Based on these results, we do not predict that either antibody would work in ICC/IF for mouse samples.
I've attached the western blot image for 2582-1 in mouse.
Answered on May 02 2013