Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
Key features and details
- Mouse monoclonal [4G11G6] to Superoxide Dismutase 3/EC-SOD
- Suitable for: Flow Cyt, ELISA, ICC/IF, IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6]
See all Superoxide Dismutase 3/EC-SOD primary antibodies -
Description
Mouse monoclonal [4G11G6] to Superoxide Dismutase 3/EC-SOD -
Host species
Mouse -
Specificity
Detects extracellular Superoxide Dismutase 3/EC-SOD.
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Tested applications
Suitable for: Flow Cyt, ELISA, ICC/IF, IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Human Superoxide Dismutase 3/EC-SOD.
Database link: P08294 -
Positive control
- Human cartilage lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4G11G6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (1) |
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
1/100.
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Notes |
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Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa). |
IHC-P
1/100. |
Target
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Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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Database links
- Entrez Gene: 6649 Human
- Entrez Gene: 20657 Mouse
- Entrez Gene: 25352 Rat
- Omim: 185490 Human
- SwissProt: P08294 Human
- SwissProt: O09164 Mouse
- SwissProt: Q08420 Rat
- Unigene: 2420 Human
see all -
Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Images
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Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
ab80946 at 1/100 dilution staining Superoxide Dismutase 3/EC-SOD in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis.
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Overlay histogram showing HEK293 cells stained with ab80946 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80946, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab80946 has been referenced in 13 publications.
- Alferiev IS et al. Stent-Based Gene Delivery for Coronary Disease. Methods Mol Biol 2573:217-233 (2022). PubMed: 36040598
- Xu D et al. Dexmedetomidine provides protection to neurons against OGD/R-induced oxidative stress and neuronal apoptosis. Toxicol Mech Methods 31:374-382 (2021). PubMed: 33648426
- Gomes-Santos IL et al. Exercise Training Preserves Myocardial Strain and Improves Exercise Tolerance in Doxorubicin-Induced Cardiotoxicity. Front Cardiovasc Med 8:605993 (2021). PubMed: 33869297
- Naguib S et al. Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension. Redox Biol 42:101883 (2021). PubMed: 33579667
- Yang X et al. Protective effect of hypoglycemic granule against diabetes-induced liver injury by alleviating glycolipid metabolic disorder and oxidative stress. J Cell Biochem 121:3221-3234 (2020). PubMed: 31894610