Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Superoxide Dismutase 3/EC-SOD antibody
See all Superoxide Dismutase 3/EC-SOD primary antibodies -
Description
Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD -
Host species
Rabbit -
Specificity
Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT. -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Superoxide Dismutase 3/EC-SOD (N terminal).
Sequence:WTGEDSAEPNSDSAEWIRD
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Positive control
- IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
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IHC-Fr |
1/200 - 1/2000.
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WB | (1) |
1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
1/200 - 1/2000. |
WB
1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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Database links
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Images
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ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
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ab21974 (1/1000) staining of human kidney tubule cells.
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All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974) at 1/500 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsSuperoxide Dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab21974 has been referenced in 12 publications.
- Alaidaroos NYA et al. Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence. Stem Cell Res Ther 12:142 (2021). PubMed: 33596998
- Peng KT et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis. PLoS One 13:e0202501 (2018). PubMed: 30125327
- Shrestha P et al. Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells. Mol Cells 39:242-9 (2016). WB . PubMed: 26912083
- Cunningham MW et al. Pregnant rats treated with a high-fat/prooxidant Western diet with ANG II and TNF-a are resistant to elevations in blood pressure and renal oxidative stress. Am J Physiol Regul Integr Comp Physiol 308:R945-56 (2015). PubMed: 25810384
- Duscher D et al. Aging disrupts cell subpopulation dynamics and diminishes the function of mesenchymal stem cells. Sci Rep 4:7144 (2014). IHC-Fr ; Mouse . PubMed: 25413454