Overview

  • Product name

    Anti-Superoxide Dismutase 3/EC-SOD antibody
    See all Superoxide Dismutase 3/EC-SOD primary antibodies
  • Description

    Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
  • Host species

    Rabbit
  • Specificity

    Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Superoxide Dismutase 3/EC-SOD (N terminal).
    Sequence:

    WTGEDSAEPNSDSAEWIRD

  • Positive control

    • IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate
  • General notes

    Previously labelled as Superoxide Dismutase 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab21974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/2000.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • Tissue specificity

    Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • Sequence similarities

    Belongs to the Cu-Zn superoxide dismutase family.
  • Cellular localization

    Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide Dismutase 3 antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Images

  • ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
  • ab21974 (1/1000) staining of human kidney tubule cells.
  • All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974) at 1/500 dilution

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    Superoxide Dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:

  • Peng KT  et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis. PLoS One 13:e0202501 (2018). Read more (PubMed: 30125327) »
  • Shrestha P  et al. Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells. Mol Cells 39:242-9 (2016). WB . Read more (PubMed: 26912083) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Citrate Buffer + 0.05% Tween 20
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde

Dr. I Zelko

Verified customer

Submitted Jan 25 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Penile Tissue)
Gel Running Conditions
Reduced Denaturing (4-15% minigel)
Loading amount
30 µg
Specification
Mouse Penile Tissue
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

Submitted Jan 25 2011

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