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Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108)

  • Datasheet
  • SDS
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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108)

    Key features and details

    • Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Superoxide Dismutase 3/EC-SOD antibody
      See all Superoxide Dismutase 3/EC-SOD primary antibodies
    • Description

      Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Rabbit
    • Immunogen

      Synthetic peptide corresponding to Superoxide Dismutase 3/EC-SOD.

    • Positive control

      • Rat and mouse Lung Tissue Extract; Human Superoxide Dismutase 3/EC-SOD Protein.
    • General notes

      Previously labelled as Superoxide Dismutase 3.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 50% Glycerol, PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Antioxidants
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Mouse lung normal tissue lysate - total protein (ab29297)
    • Recombinant Protein

      • Recombinant Human Superoxide Dismutase 3/EC-SOD protein (His tag) (ab236182)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab83108 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).

    Target

    • Function

      Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
    • Tissue specificity

      Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
    • Sequence similarities

      Belongs to the Cu-Zn superoxide dismutase family.
    • Cellular localization

      Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
    • Target information above from: UniProt accession P08294 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6649 Human
      • Entrez Gene: 20657 Mouse
      • Entrez Gene: 25352 Rat
      • Omim: 185490 Human
      • SwissProt: P08294 Human
      • SwissProt: O09164 Mouse
      • SwissProt: P41975 Rabbit
      • SwissProt: Q08420 Rat
      • Unigene: 2420 Human
      • Unigene: 2407 Mouse
      • Unigene: 10358 Rat
      see all
    • Alternative names

      • EC SOD antibody
      • EC-SOD antibody
      • Extracellular superoxide dismutase [Cu Zn] antibody
      • Extracellular superoxide dismutase [Cu-Zn] antibody
      • Extracellular superoxide dismutase antibody
      • Extracellular superoxide dismutase precursor antibody
      • MGC20077 antibody
      • SOD 3 antibody
      • SOD3 antibody
      • SODE_HUMAN antibody
      • Superoxide Dismutase 3 antibody
      • Superoxide dismutase 3 extracellular antibody
      see all

    Images

    • Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108)
      Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108)
      All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108) at 1/1000 dilution

      Lane 1 : Rat lung tissue extract
      Lane 2 : Mouse lung tissue extract
      Lane 3 : Human Superoxide Dismutase 3/EC-SOD protein

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?
      Additional bands at: 40 kDa (possible glycosylated form), 60 kDa (possible dimer)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab83108? Please let us know so that we can cite the reference in this datasheet.

    ab83108 has been referenced in 3 publications.

    • Sulistyowati E  et al. Indonesian herbal medicine prevents hypertension-induced left ventricular hypertrophy by diminishing NADPH oxidase-dependent oxidative stress. Oncotarget 8:86784-86798 (2017). PubMed: 29156835
    • Diao Z  et al. Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90 (2017). PubMed: 28189851
    • Ramiro-Diaz JM  et al. Mechanisms of NFATc3 activation by increased superoxide and reduced hydrogen peroxide in pulmonary arterial smooth muscle. Am J Physiol Cell Physiol 307:C928-38 (2014). PubMed: 25163518

    Customer reviews and Q&As

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