Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Superoxide Dismutase 3/EC-SOD antibody
See all Superoxide Dismutase 3/EC-SOD primary antibodies -
Description
Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit
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Immunogen
Synthetic peptide corresponding to Superoxide Dismutase 3/EC-SOD.
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Positive control
- Rat and mouse Lung Tissue Extract; Human Superoxide Dismutase 3/EC-SOD Protein.
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General notes
Previously labelled as Superoxide Dismutase 3.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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Database links
- Entrez Gene: 6649 Human
- Entrez Gene: 20657 Mouse
- Entrez Gene: 25352 Rat
- Omim: 185490 Human
- SwissProt: P08294 Human
- SwissProt: O09164 Mouse
- SwissProt: P41975 Rabbit
- SwissProt: Q08420 Rat
see all -
Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Images
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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108)All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108) at 1/1000 dilution
Lane 1 : Rat lung tissue extract
Lane 2 : Mouse lung tissue extract
Lane 3 : Human Superoxide Dismutase 3/EC-SOD protein
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible glycosylated form), 60 kDa (possible dimer)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab83108 has been referenced in 3 publications.
- Sulistyowati E et al. Indonesian herbal medicine prevents hypertension-induced left ventricular hypertrophy by diminishing NADPH oxidase-dependent oxidative stress. Oncotarget 8:86784-86798 (2017). PubMed: 29156835
- Diao Z et al. Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90 (2017). PubMed: 28189851
- Ramiro-Diaz JM et al. Mechanisms of NFATc3 activation by increased superoxide and reduced hydrogen peroxide in pulmonary arterial smooth muscle. Am J Physiol Cell Physiol 307:C928-38 (2014). PubMed: 25163518