Product nameAnti-Superoxide Dismutase 3/EC-SOD antibody
See all Superoxide Dismutase 3/EC-SOD primary antibodies
DescriptionRabbit polyclonal to Superoxide Dismutase 3/EC-SOD
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in the following human tissue lysates: Artery; Heart; Lung; Kidney.
Previously labelled as Superoxide Dismutase 3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab90258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).|
FunctionProtect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Tissue specificityExpressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
Cellular localizationSecreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
- Information by UniProt
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258) at 1 µg/ml
Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Lung (Human) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32,35 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 42 kDa, 50 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Superoxide dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt), which may explain the migration pattern observed.