Overview

  • Product name

    Anti-Superoxide Dismutase 3/EC-SOD antibody
    See all Superoxide Dismutase 3/EC-SOD primary antibodies
  • Description

    Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Superoxide Dismutase 3/EC-SOD aa 1-100 conjugated to keyhole limpet haemocyanin.

    Read Abcam's proprietary immunogen policy
    (Peptide available as ab99867)

  • Positive control

    • This antibody gave a positive signal in the following human tissue lysates: Artery; Heart; Lung; Kidney.
  • General notes

    Previously labelled as Superoxide Dismutase 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab90258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • Tissue specificity

    Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • Sequence similarities

    Belongs to the Cu-Zn superoxide dismutase family.
  • Cellular localization

    Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide Dismutase 3 antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Images

  • All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258) at 1 µg/ml

    Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 32,35 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 42 kDa, 50 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    Superoxide dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt), which may explain the migration pattern observed.

References

This product has been referenced in:

  • Katamune C  et al. Mutation of the gene encoding the circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulating the Aldh3a1 gene. J Biol Chem 294:547-558 (2019). Read more (PubMed: 30429219) »
See 1 Publication for this product

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