Recombinant Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (ab249643)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10860] to Superoxide Dismutase 3/EC-SOD - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free
See all Superoxide Dismutase 3/EC-SOD primary antibodies -
Description
Rabbit monoclonal [EPR10860] to Superoxide Dismutase 3/EC-SOD - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Superoxide Dismutase 3/EC-SOD aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P08294 -
General notes
ab249643 is the carrier-free version of ab171738.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10860 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. |
Target
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Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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Database links
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Images
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This data was developed using ab171738, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human kidney tissue labeling Superoxide Dismutase 3/EC-SOD using ab171738 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab171738, the same antibody clone in a different buffer formulation.Immunohistochemistry of paraffin-embedded Human heart tissue labeling Superoxide Dismutase 3/EC-SOD using ab171738 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab171738, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249643 has not yet been referenced specifically in any publications.