Recombinant
RabMAb

Recombinant Anti-Superoxide Dismutase 4/CCS antibody [EPR9712] (ab167170)

Overview

  • Product name

    Anti-Superoxide Dismutase 4/CCS antibody [EPR9712]
    See all Superoxide Dismutase 4/CCS primary antibodies
  • Description

    Rabbit monoclonal [EPR9712] to Superoxide Dismutase 4/CCS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Superoxide Dismutase 4/CCS.
    Database link: O14618

  • Positive control

    • Jurkat, HepG2 and HeLa cell lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Superoxide Dismutase 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Delivers copper to copper zinc superoxide dismutase (SOD1).
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.
      Contains 1 HMA domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • CCS antibody
      • CCS_HUMAN antibody
      • Copper chaperone for superoxide dismutase antibody
      • MGC138260 antibody
      • SOD 4 antibody
      • SOD4 antibody
      • Superoxide dismutase copper chaperone antibody
      see all

    Images

    • All lanes : Anti-Superoxide Dismutase 4/CCS antibody [EPR9712] (ab167170) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 & 3 : HRP labeled goat anti-rabbit at 1/2000 dilution
      Lane 2 : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunofluorescence analysis of HepG2 cells labeling Superoxide Dismutase 4/CCS with ab167170 at 1/100 dilution.

    References

    This product has been referenced in:

    • Li Y  et al. Copper Chaperone for Superoxide Dismutase Promotes Breast Cancer Cell Proliferation and Migration via ROS-Mediated MAPK/ERK Signaling. Front Pharmacol 10:356 (2019). Read more (PubMed: 31024318) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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