Recombinant
RabMAb

Recombinant Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)] (ab137131)

Overview

  • Product name

    Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)]
    See all Superoxide Dismutase 4/CCS primary antibodies
  • Description

    Rabbit monoclonal [EPR9713(B)] to Superoxide Dismutase 4/CCS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Superoxide Dismutase 4/CCS aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, HepG2, and Jurkat cell lysates
  • General notes

     

     

     This product was previously labelled as Superoxide Dismutase 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    Images

    • All lanes : Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)] (ab137131) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 29 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?

    References

    ab137131 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 21 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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