Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

Submit an Abreview Q&A (25)Specific References (24)

Overview

  • Product name
    Superoxide Dismutase Activity Assay Kit (Colorimetric)
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Enzyme activity
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    SOD Assay Buffer WM 1 x 20ml
    SOD Dilution Buffer NM 1 x 10ml
    SOD Enzyme Solution Green 1 x 20µl
    WST Solution Red 1 x 1ml
  • Research areas
  • Relevance
    Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3).
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • ALS
    • ALS1
    • Cu /Zn superoxide dismutase
    • Cu/Zn superoxide dismutase
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase cystolic
    see all

Associated products

Images

  • Functional studies - ab65354
    Functional studies - ab65354Image from Park J et al., PLoS One 12(2), fig 4b. Doi: 10.1371/journal.pone.0172751

    Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).

    * Indicates statistical significance in comparison with DM group (P < 0.05). # Indicates statistical significance in comparison with the statin group (P<0.05).

  • Functional Studies- Superoxide Dismutase Activity Assay Kit (ab65354)
    Functional Studies- Superoxide Dismutase Activity Assay Kit (ab65354)

    Superoxide dismutase measured in biofluids at various dilutions

  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
    Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

    Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)

  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
    Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
    Principle of Superoxide Dismutase Assay.

Protocols

References

This product has been referenced in:
  • Hai B  et al. Delivery of Sonic Hedgehog Gene Repressed Irradiation-induced Cellular Senescence in Salivary Glands by Promoting DNA Repair and Reducing Oxidative Stress. Theranostics 8:1159-1167 (2018). Read more (PubMed: 29464006) »
  • Belhadj S  et al. Beneficial Effect of Jojoba Seed Extracts on Hyperglycemia-Induced Oxidative Stress in RINm5f Beta Cells. Nutrients 10:N/A (2018). Read more (PubMed: 29558444) »

See all 24 Publications for this product

Publishing research using ab65354? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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25 reviews or Q&A

10
Votes: Highest First

1. Can we do the test without SOD standard? Do you have some explanation how we should do the test if we dont have the SOD standard

2. If we should order the SOD how much the price and how long it takes to order.

The ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit can be used without a standard since it is reporting as % inhibition in your sample. Blank 1 does not contain any inhibition, and your sample will have some percentage of inhibition. Bla...

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Is it possible to prolong the shelf life of product ab65354 by storing WST Working Solution in different conditions than the 4C stated in the protocol after reconstitution?

I would not suggest this, as we store this product at 4C ourselves. We haven't tried to store this productat -20C, and we do not know if the product is suitable to be stored at lower temperatures. Please be aware that the customer will loose the Abprom...

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Hi, Can you please tell me what is the lower limit of detection for this assay? Thank you!

The lower limit for this kit is 0.1 U/ml of SOD activity.

We calculated the results as inhibition rate%. To calculate the SOD activity in U/ml, should we use the standard or is there any conversion factor for this?

This kit is not originally designed to be run with a standard curve each time. However, if you wish to run a standard curve to calculate absolute activity (the assay is designed to calculate percent inhibition with respect to control sample), you can t...

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Which one of the three isoforms of SOD is directed for in the kit?

I can confirm that there is nothing isoform-specific in the kit principle. ab65354 measures the oxygen anion created by any of the SOD enzymes.

What mass of tissue is recommended as starting material for this assay?

The minimum mass of tissue to use with this assay is 10mg.

Could you please advise me, should the protein concentration be measured of the samples tested? The protocol you have provided shows a graph of percent inhibition vs SOD activity, has this been normalised to the protein concentration?

The graph we have on our datasheet has the % inhibition on Y-axis. From this kit protocol, you will just get the Y-value which is the % inhibition. To draw the graph, you will need the X value. You can get that by using standards (not included in the k...

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How much tissue should be used for extraction?

We would recommend using at least 10 mg of tissue with the kit ab65354.

How do we prepare blood samples to use with this kit?

Collect using citrate or EDTA. Centrifuge at 1,000 x g for 10 min at 4°C. Remove plasma layer w/o disturbing the buffy layer and store at -80°C until ready for analysis. Remove the buffy layer from the red cell pellet. Resuspend erythrocytes in 5x volu...

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Are three blanks required for each sample, or can three blanks suffice for comparison to all samples run at the same time?

Yes, you would need the 3 controls with each and every sample.

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