Overview

  • Product name
    Superoxide Dismutase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Enzyme activity
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.


    In the SOD assay protocol:
    - superoxide anions are produced by the action of xanthine oxidase
    - SOD catalyzes the dismutation of the superoxide anion into hydrogen peroxide and O2
    - superoxide anions act on WST-1 to produce a water-soluble formazan dye which can be detected by the increase in absorbance at 450 nm
    The greater the activity of SOD in the sample, the less formazan dye is produced.


    Superoxide dismutase assay protocol summary:
    - add samples to wells
    - add WST-1 working solution and enzyme working solution and incubate for 20 min at 37ºC
    - analyze with microplate reader

  • Notes

    Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    SOD Assay Buffer WM 1 x 20ml
    SOD Dilution Buffer NM 1 x 10ml
    SOD Enzyme Solution Green 1 x 20µl
    WST Solution Red 1 x 1ml
  • Research areas
  • Relevance
    Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3).
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • ALS
    • ALS1
    • Cu /Zn superoxide dismutase
    • Cu/Zn superoxide dismutase
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase cystolic
    see all

Associated products

Images

  • Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).

    * Indicates statistical significance in comparison with DM group (P < 0.05). Indicates statistical significance in comparison with the statin group (P<0.05).

  • Superoxide dismutase measured in biofluids at various dilutions

  • Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)

  • Principle of Superoxide Dismutase Assay.

Protocols

References

This product has been referenced in:
  • Cabrejos DAL  et al. Structural characterization of a pathogenicity-related superoxide dismutase codified by a probably essential gene in Xanthomonas citri subsp. citri. PLoS One 14:e0209988 (2019). Read more (PubMed: 30615696) »
  • Kaushal JB  et al. Sonic hedgehog protects endometrial hyperplasial cells against oxidative stress via suppressing mitochondrial fission protein dynamin-like GTPase (Drp1). Free Radic Biol Med 129:582-599 (2018). Read more (PubMed: 30347228) »
See all 35 Publications for this product

Customer reviews and Q&As

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Abreviews
The measurement of Superoxide Dismutase (SOD) activity in bacterial supernatants was performed. Bacterial cells were grown in defined medium and harvested at late exponential phase by centrifugation at 15,000 x g for 20 min. The obtained supernatant was sterile filtrated using a 0.22 µm filter. The assay procedure was done according to manufacture‘s instruction for colorimetric assays. We compared to different mutants with the wildtype strain. Unlike the catalase activity (see catalse acitivity assay from Abcam [ab83464]) there was no significant difference in the activity of secreted SOD.

Miss. Lisa Teubner

Verified customer

Submitted Mar 18 2019

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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