Key features and details
- Rabbit polyclonal to SUPT16H
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SUPT16H antibody
See all SUPT16H primary antibodies
DescriptionRabbit polyclonal to SUPT16H
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis
- Human rectum tissue; RT-4 and U-251 MG cell lysates, Human plasma and Human tonsil tissue lysates; U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 120 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Sequence similaritiesBelongs to the peptidase M24 family. SPT16 subfamily.
DomainThe Glu-rich acidic region in C-terminus is essential for FACT activity.
modificationsADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
- Information by UniProt
- CDC68 antibody
- Chromatin specific transcription elongation factor 140 kDa subunit antibody
- Chromatin-specific transcription elongation factor 140 kDa subunit antibody
Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling SUPT16H with ab204343 at 1/1000 dilution.
All lanes : Anti-SUPT16H antibody (ab204343) at 1/500 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 120 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling SUPT16H with ab204343 at 4 µg/ml (green).
ab204343 has been referenced in 1 publication.
- Porter LF et al. A role for repressive complexes and H3K9 di-methylation in PRDM5-associated brittle cornea syndrome. Hum Mol Genet 24:6565-79 (2015). IHC-P ; Human . PubMed: 26395458