• Product name

    Anti-SUPT16H antibody [EPR3685]
    See all SUPT16H primary antibodies
  • Description

    Rabbit monoclonal [EPR3685] to SUPT16H
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SUPT16H. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat and A549 cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 120 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • Tissue specificity

    • Sequence similarities

      Belongs to the peptidase M24 family. SPT16 subfamily.
    • Domain

      The Glu-rich acidic region in C-terminus is essential for FACT activity.
    • Post-translational

      ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
    • Cellular localization

      Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
    • Information by UniProt
    • Database links

    • Alternative names

      • CDC68 antibody
      • Chromatin specific transcription elongation factor 140 kDa subunit antibody
      • Chromatin-specific transcription elongation factor 140 kDa subunit antibody
      • Facilitates chromatin transcription complex subunit SPT16 antibody
      • FACT 140 kDa subunit antibody
      • FACT antibody
      • FACT complex subunit SPT16 antibody
      • FACTp140 antibody
      • FLJ10857 antibody
      • FLJ14010 antibody
      • hSPT16 antibody
      • SP16H_HUMAN antibody
      • Suppressor of Ty 16 homolog antibody
      • Supt16h antibody
      see all


    • All lanes : Anti-SUPT16H antibody [EPR3685] (ab108960) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 120 kDa


    ab108960 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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