Key features and details
- Mouse monoclonal [3079C1a] to SUPT4H
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-SUPT4H antibody [3079C1a]
See all SUPT4H primary antibodies
DescriptionMouse monoclonal [3079C1a] to SUPT4H
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SUPT4H (internal sequence).
This product was previously labelled as Suppressor of Ty 4 homolog 1
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab54350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionComponent of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the SPT4 family.
- Information by UniProt
- DRB sensitivity-inducing factor 14 kDa subunit antibody
- DRB sensitivity-inducing factor small subunit antibody
- DSIF p14 antibody
Anti-SUPT4H antibody [3079C1a] (ab54350) at 1/100 dilution + immunising recombinant protein
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size: 13 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
The immunogen peptide in this image was tagged. Secondary antibody: Sheep Anti Mouse IgG (Original Conc.; Antibody:0.63mg/ml, HRP: 0.78mg/ml) Immunogen amount: 25ng/lane. MW of Tag: approx. 20kDa.
Antibody only tested on recombinant fragment as immunogen. We have no data on detection of endogenous protein.
ab54350 has been referenced in 1 publication.
- Liu CR et al. Spt4 is selectively required for transcription of extended trinucleotide repeats. Cell 148:690-701 (2012). PubMed: 22341442