Overview

  • Product name

    Anti-SUPT4H antibody [3079C1a]
    See all SUPT4H primary antibodies
  • Description

    Mouse monoclonal [3079C1a] to SUPT4H
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SUPT4H (internal sequence).

  • General notes

     This product was previously labelled as Suppressor of Ty 4 homolog 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab54350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the SPT4 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DRB sensitivity-inducing factor 14 kDa subunit antibody
    • DRB sensitivity-inducing factor small subunit antibody
    • DSIF p14 antibody
    • DSIF small subunit antibody
    • hSPT4 antibody
    • SPT4 antibody
    • SPT4H antibody
    • SPT4H_HUMAN antibody
    • SUPT4H `1 antibody
    • SUPT4H antibody
    • Supt4h1 antibody
    • Transcription elongation factor spt4 antibody
    see all

Images

  • Anti-SUPT4H antibody [3079C1a] (ab54350) at 1/100 dilution + immunising recombinant protein

    Secondary
    Sheep Anti Mouse IgG at 1/3000 dilution

    Predicted band size: 13 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    The immunogen peptide in this image was tagged. Secondary antibody: Sheep Anti Mouse IgG (Original Conc.; Antibody:0.63mg/ml, HRP: 0.78mg/ml) Immunogen amount: 25ng/lane. MW of Tag: approx. 20kDa.

    Antibody only tested on recombinant fragment as immunogen. We have no data on detection of endogenous protein.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Answer

Merci de nous avoir contactés.

La bande obtenue avec le ab54530 correspond à la protéine ayant servi comme immunogène (13 kDa)et non à la protéine endogène (32 kDa).
https://www.abcam.com/suppressor-of-ty-4-homolog-1-antibody-3079c1a-ab54350.html#ab54350.jpg

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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