RecombinantRabMAb

Recombinant Anti-SUPT4H antibody [EPR6100(2)] (ab129187)

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Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
  • OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
  • Anti-SUPT4H antibody [EPR6100(2)] (ab129187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6100(2)] to SUPT4H
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SUPT4H antibody [EPR6100(2)]
    See all SUPT4H primary antibodies

  • Description

    Rabbit monoclonal [EPR6100(2)] to SUPT4H

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human SUPT4H aa 50-150.
    Database link: P63272

  • Positive control

    • 293T and MCF-7 cell lysates

  • General notes

     

     

     This product was previously labelled as Suppressor of Ty 4 homolog 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab129187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the SPT4 family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DRB sensitivity-inducing factor 14 kDa subunit antibody
      • DRB sensitivity-inducing factor small subunit antibody
      • DSIF p14 antibody
      • DSIF small subunit antibody
      • hSPT4 antibody
      • SPT4 antibody
      • SPT4H antibody
      • SPT4H_HUMAN antibody
      • SUPT4H `1 antibody
      • SUPT4H antibody
      • Supt4h1 antibody
      • Transcription elongation factor spt4 antibody
      see all

    Images

    • Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
      Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
      All lanes : Anti-SUPT4H antibody [EPR6100(2)] (ab129187) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 13 kDa

    • OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
      OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-SUPT4H antibody [EPR6100(2)] (ab129187)
      Anti-SUPT4H antibody [EPR6100(2)] (ab129187)

    References (0)

    Publishing research using ab129187? Please let us know so that we can cite the reference in this datasheet.

    ab129187 has not yet been referenced specifically in any publications.

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