• Product name
  • Description
    Rabbit polyclonal to SUPT5H
  • Host species
  • Specificity
    ab26259 recognises SUPT5H protein.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within Human SUPT5H aa 892-941 (C terminal). The exact sequence is proprietary.


    Database link: O00267

  • Positive control
    • HepG2 cell lysate, human kidney tissue, human pancreas tissue


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab26259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • Function
    Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SPT5 family.
    Contains 5 KOW domains.
  • Post-translational
    Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
    Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DRB sensitivity inducing factor large subunit antibody
    • DRB sensitivity-inducing factor 160 kDa subunit antibody
    • DRB sensitivity-inducing factor large subunit antibody
    • DSIF large subunit antibody
    • DSIF p160 antibody
    • FLJ34157 antibody
    • hSPT 5 antibody
    • hSPT5 antibody
    • SPT 5 antibody
    • SPT 5H antibody
    • SPT5 antibody
    • SPT5H antibody
    • SPT5H_HUMAN antibody
    • Suppressor of Ty 5 homolog antibody
    • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
    • SUPT 5H antibody
    • supt5h antibody
    • Tat cotransactivator 1 protein antibody
    • Tat CT1 antibody
    • Tat CT1 protein antibody
    • Tat-cotransactivator 1 protein antibody
    • Tat-CT1 protein antibody
    • Transcription elongation factor SPT 5 antibody
    • Transcription elongation factor spt5 antibody
    • Transcription factor Tat CT1 antibody
    see all


  • Anti-SUPT5H antibody (ab26259) at 0.5 µg/ml + HepG2 cell lysate

    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size: 121 kDa

  • Staining of human kidney tissue with ab26259 (4-8 ug/ml). Positive labelled epithelial cells of renal tubule are indicated with arrows. 400X magnification.
  • Staining of human pancreas tissue with ab26259 (4-8 ug/ml). Positive labelled epithelial cells of pancreatic acinus are indicated with arrows. 400X magnification.


This product has been referenced in:
  • Zhao Z  et al. CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes. J Biol Chem 292:15489-15500 (2017). Read more (PubMed: 28743741) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. I have checked our catalog and have gathered the following information: 1. STE12: We don't have antibodies to this target protein in our catalog. 2. TEC1(ROC1): ab2977 has 100% homology - and qualifi...

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I can confirm that the antibody from BD is not ours however I'm afraid we are unable to find out the sequence as it is commercially sensitive. As we have not done any comparison with others that displays 160 kDa we have no explanation for it I'm afrai...

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