Overview

  • Product name

  • Description

    Rabbit polyclonal to SUR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to SUR1 aa 1560-1582.

Properties

Applications

Our Abpromise guarantee covers the use of ab32844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20304763
ELISA 1/10000 - 1/100000.
WB 1/500. Predicted molecular weight: 175 kDa.

Target

  • Function

    Putative subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release.
  • Involvement in disease

    Defects in ABCC8 are a cause of leucine-induced hypoglycemia (LIH) [MIM:240800]; also known as leucine-sensitive hypoglycemia of infancy. LIH is a rare cause of hypoglycemia and is described as a condition in which symptomatic hypoglycemia is provoked by high protein feedings. Hypoglycemia is also elicited by administration of oral or intravenous infusions of a single amino acid, leucine.
    Defects in ABCC8 are the cause of familial hyperinsulinemic hypoglycemia type 1 (HHF1) [MIM:256450]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
    Defects in ABCC8 are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in ABCC8 are the cause of transient neonatal diabetes mellitus type 2 (TNDM2) [MIM:610374]. Neonatal diabetes is a form of diabetes mellitus defined by the onset of mild-to-severe hyperglycemia within the first months of life. Transient neonatal diabetes remits early, with a possible relapse during adolescence.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC36 antibody
    • Abcc8 antibody
    • ABCC8_HUMAN antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 8 antibody
    • ATP binding cassette transporter sub family C member 8 (1) antibody
    • ATP-binding cassette sub-family C member 8 antibody
    • HHF1 antibody
    • HI antibody
    • HRINS antibody
    • MRP8 antibody
    • PHHI antibody
    • Sulfonylurea receptor (hyperinsulinemia) antibody
    • Sulfonylurea receptor 1 antibody
    • SUR antibody
    • SUR1 antibody
    • SUR1delta2 antibody
    • TNDM2 antibody
    see all

References

This product has been referenced in:

  • Zhou F  et al. Neuroprotective potential of glibenclamide is mediated by antioxidant and anti-apoptotic pathways in intracerebral hemorrhage. Brain Res Bull 142:18-24 (2018). Read more (PubMed: 29933037) »
  • Xu S  et al. Autocrine insulin increases plasma membrane KATP channel via PI3K-VAMP2 pathway in MIN6 cells. Biochem Biophys Res Commun 468:752-7 (2015). WB ; Mouse . Read more (PubMed: 26585489) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

We now have new lot in stock so I can send you one vial as a replacement antibody. Again because we haven't tested this lot in mouse so we cannot confirm the ab will work.

You have kindly mentioned in previous emails that the antibody worked before so perhaps running suggested human lysates will give goodindication whether the antibody is wrong or the lysates.

Let me know if you are interested in getting the new lot.

Read More

Question
Answer

Thank you for confirming all the details.

The mouse species has been added to the datasheet from a publication http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20362548&dopt=Abstract. We unfortunately hold no tested data with mouse lysates. The mouse and human SUR1 are quite similar at the immunogenic region so it is more likely that the antibody will work.

We currently haven't received any complaint other than the 2 you submitted last year. So we are not sure if this issue is lot related. I have checked the case again and would like to suggest changing few things and hoping that the antibody will be OK;

- SUR1 is multipass membrane protein; for mmp we do not recommend boiling the sample with loading buffer. Please try heating samples for 60-70C for 10-15 minutes.
- The lot conc. we have sent is 0.5 mg/ml. 1/100 dilution is OK to use.
- if possible please include a positive control with human cell lines e.g. U2OS, A431, pancreatic tissue lysates, Tonsil, gall bladder, liver or lung tissue lysates.

Please address all the emails of this complaint to me (ab32844).

Let me know how these suggestions go. if the results does not improve I will then ask to choose another anti SUR1 antibody.

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

PS:Please provide all the details of experiemtn andany troubleshooting you have already done! Thanks. Please do not forget to add the purchase order number.

Read More

Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply. PS: Please include images for each antibody also. Images always helps in understanding the problem quickly.

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