Overview

  • Product name

    Anti-SUR1 antibody [N289/16]
    See all SUR1 primary antibodies
  • Description

    Mouse monoclonal [N289/16] to SUR1
  • Host species

    Mouse
  • Specificity

    Does not cross react with SUR2B.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster
  • Immunogen

    His-tag fusion protein, corresponding to cytoplasmic, C terminal amino acids 1548-1582 of Rat SUR1.

  • Positive control

    • WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S289-16 to N289/16, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab134292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 177 kDa.

1µg/ml of ab134292 is sufficient for detection of SUR1 in 20µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects a band at ~160kDa.

IHC-P Use at an assay dependent dilution.
ICC/IF 1/100.

Target

  • Function

    Putative subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release.
  • Involvement in disease

    Defects in ABCC8 are a cause of leucine-induced hypoglycemia (LIH) [MIM:240800]; also known as leucine-sensitive hypoglycemia of infancy. LIH is a rare cause of hypoglycemia and is described as a condition in which symptomatic hypoglycemia is provoked by high protein feedings. Hypoglycemia is also elicited by administration of oral or intravenous infusions of a single amino acid, leucine.
    Defects in ABCC8 are the cause of familial hyperinsulinemic hypoglycemia type 1 (HHF1) [MIM:256450]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
    Defects in ABCC8 are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in ABCC8 are the cause of transient neonatal diabetes mellitus type 2 (TNDM2) [MIM:610374]. Neonatal diabetes is a form of diabetes mellitus defined by the onset of mild-to-severe hyperglycemia within the first months of life. Transient neonatal diabetes remits early, with a possible relapse during adolescence.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC36 antibody
    • Abcc8 antibody
    • ABCC8_HUMAN antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 8 antibody
    • ATP binding cassette transporter sub family C member 8 (1) antibody
    • ATP-binding cassette sub-family C member 8 antibody
    • HHF1 antibody
    • HI antibody
    • HRINS antibody
    • MRP8 antibody
    • PHHI antibody
    • Sulfonylurea receptor (hyperinsulinemia) antibody
    • Sulfonylurea receptor 1 antibody
    • SUR antibody
    • SUR1 antibody
    • SUR1delta2 antibody
    • TNDM2 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for SUR1 (green) using ab134292 at 1/100 dilution in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes room temperature, 5 minutes room temperature.

    (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134292. (D) Composite.

  • Anti-SUR1 antibody [N289/16] (ab134292) at 1/200 dilution + Rat brain membrane lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG: HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 177 kDa

References

ab134292 has not yet been referenced specifically in any publications.

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