SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)

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Key features and details

  • SureLight® APC Mouse monoclonal [9E10] to Myc tag
  • Suitable for: Flow Cyt, FRET
  • Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
  • Isotype: IgG1

Overview

  • Product name

    SureLight® APC Anti-Myc tag antibody [9E10]
    See all Myc tag primary antibodies

  • Description

    SureLight® APC Mouse monoclonal [9E10] to Myc tag

  • Host species

    Mouse

  • Conjugation

    SureLight® APC. Ex: 652nm, Em: 657nm

  • Specificity

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.

  • Tested applications

    Suitable for: Flow Cyt, FRETmore details

  • Immunogen

    Synthetic peptide: AEEQKLISEE DLLRKRREQL KHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.

  • Epitope

    ab72580 recognizes the 9E10 epitope (EQKLISEEDL sequence) derived from the human c-myc protein.

  • General notes

    Excitation max. 652 nm. Emission max. 657.5 nm. F/P 1:1.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab72580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FRET Use at an assay dependent concentration.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • Cellular localization

    Nuclear

  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

References (2)

Publishing research using ab72580? Please let us know so that we can cite the reference in this datasheet.

ab72580 has been referenced in 2 publications.

  • Hörnström D  et al. Molecular optimization of autotransporter-based tyrosinase surface display. Biochim Biophys Acta Biomembr 1861:486-494 (2019). PubMed: 30521785
  • Gustavsson M  et al. Surface expression of ?-transaminase in Escherichia coli. Appl Environ Microbiol 80:2293-8 (2014). PubMed: 24487538

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