SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
Key features and details
- SureLight® APC Mouse monoclonal [9E10] to Myc tag
- Suitable for: Flow Cyt, FRET
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG1
Overview
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Product name
SureLight® APC Anti-Myc tag antibody [9E10]
See all Myc tag primary antibodies -
Description
SureLight® APC Mouse monoclonal [9E10] to Myc tag -
Host species
Mouse -
Conjugation
SureLight® APC. Ex: 652nm, Em: 657nm -
Specificity
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
Tested applications
Suitable for: Flow Cyt, FRETmore details -
Immunogen
Synthetic peptide: AEEQKLISEE DLLRKRREQL KHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.
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Epitope
ab72580 recognizes the 9E10 epitope (EQKLISEEDL sequence) derived from the human c-myc protein. -
General notes
Excitation max. 652 nm. Emission max. 657.5 nm. F/P 1:1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 8.20
Preservative: 0.013% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA, 0.87% Sodium chloride, 97.37% Tris buffered saline -
Concentration information loading...
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Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
Clonality
Monoclonal -
Clone number
9E10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
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Assay kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab72580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | Use at an assay dependent concentration. ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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FRET | Use at an assay dependent concentration. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
References (2)
ab72580 has been referenced in 2 publications.
- Hörnström D et al. Molecular optimization of autotransporter-based tyrosinase surface display. Biochim Biophys Acta Biomembr 1861:486-494 (2019). PubMed: 30521785
- Gustavsson M et al. Surface expression of ?-transaminase in Escherichia coli. Appl Environ Microbiol 80:2293-8 (2014). PubMed: 24487538