SureLight® APC Anti-T7 tag® antibody (ab72565)
Key features and details
- SureLight® APC Rabbit polyclonal to T7 tag®
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG
Overview
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Product name
SureLight® APC Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
SureLight® APC Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
SureLight® APC. Ex: 652nm, Em: 657nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide T7 tag conjugated to a carrier molecule
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General notes
Excitation maximum:650 nm Emission maximum:660 nm
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials werer used. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab72565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent dilution. |
Target
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Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Images
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Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (ab72565)
Flow cytometry analysis of a recombinant (N-terminus T7-tag) transmembrane cell surface receptor in CHO cells using ab72565 at a 1/20 dilution (yellow). Black - Mouse IgG SureLight® Allophycocyanin isotype control.
References (1)
ab72565 has been referenced in 1 publication.
- de la Fuente M et al. Mapping human protease-activated receptor 4 (PAR4) homodimer interface to transmembrane helix 4. J Biol Chem 287:10414-23 (2012). Flow Cyt . PubMed: 22318735
