SureLight® APC Anti-T7 tag® antibody (ab72565)
Key features and details
- SureLight® APC Rabbit polyclonal to T7 tag®
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG
Overview
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Product name
SureLight® APC Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
SureLight® APC Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
SureLight® APC. Ex: 652nm, Em: 657nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide T7 tag conjugated to a carrier molecule
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General notes
Excitation maximum:650 nm Emission maximum:660 nm
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials werer used. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent dilution.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent dilution. |
Target
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Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Images
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Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (ab72565)
Flow cytometry analysis of a recombinant (N-terminus T7-tag) transmembrane cell surface receptor in CHO cells using ab72565 at a 1/20 dilution (yellow). Black - Mouse IgG SureLight® Allophycocyanin isotype control.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72565 has been referenced in 1 publication.
- de la Fuente M et al. Mapping human protease-activated receptor 4 (PAR4) homodimer interface to transmembrane helix 4. J Biol Chem 287:10414-23 (2012). Flow Cyt . PubMed: 22318735