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    surelight-apc-t7-tag-antibody-ab72565.pdf

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Tags & Cell Markers Epitope Tags T7 Tag
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SureLight® APC Anti-T7 tag® antibody (ab72565)

  • Datasheet
  • SDS
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Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (ab72565)

    Key features and details

    • SureLight® APC Rabbit polyclonal to T7 tag®
    • Suitable for: Flow Cyt
    • Reacts with: Species independent
    • Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
    • Isotype: IgG

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    Overview

    • Product name

      SureLight® APC Anti-T7 tag® antibody
      See all T7 tag® primary antibodies
    • Description

      SureLight® APC Rabbit polyclonal to T7 tag®
    • Host species

      Rabbit
    • Conjugation

      SureLight® APC. Ex: 652nm, Em: 657nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide T7 tag conjugated to a carrier molecule

    • General notes

      Excitation maximum:650 nm Emission maximum:660 nm

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.013% Sodium azide
      Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose
    • Concentration information loading...
    • Purity

      Size exclusion
    • Purification notes

      Size exclusion chromatography as well as some proprietary methods for purifying the final materials werer used.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • T7 Tag

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab72565 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent dilution.
    Notes
    Flow Cyt
    Use at an assay dependent dilution.

    Target

    • Relevance

      The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
    • Alternative names

      • 10 antibody
      • Major capsid protein 10A antibody
      • Major capsid protein antibody
      • Minor capsid protein 10B antibody
      • T7 epitope tag antibody
      see all

    Images

    • Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (ab72565)
      Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (ab72565)

      Flow cytometry analysis of a recombinant (N-terminus T7-tag) transmembrane cell surface receptor in CHO cells using ab72565 at a 1/20 dilution (yellow). Black - Mouse IgG SureLight® Allophycocyanin isotype control.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab72565? Please let us know so that we can cite the reference in this datasheet.

    ab72565 has been referenced in 1 publication.

    • de la Fuente M  et al. Mapping human protease-activated receptor 4 (PAR4) homodimer interface to transmembrane helix 4. J Biol Chem 287:10414-23 (2012). Flow Cyt . PubMed: 22318735

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