Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)](https://www.abcam.com/ps/products/51/ab51891/Images/ab51891-63959-anti-surfactant-protein-a-antibody-6f10-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)")
Key features and details
- Mouse monoclonal [6F10] to Surfactant Protein A/PSAP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Surfactant Protein A/PSAP antibody [6F10]
See all Surfactant Protein A/PSAP primary antibodies -
Description
Mouse monoclonal [6F10] to Surfactant Protein A/PSAP -
Host species
Mouse -
Specificity
ab51891 is specific for human pulmonary Surfactant Protein A/PSAP.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length native protein (purified) corresponding to Human Surfactant Protein A/PSAP.
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Positive control
- Human lung tissue
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General notes
This product was previously labelled as Surfactant Protein A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6F10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Predicted molecular weight: 26 kDa.
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 26 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
Target
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Cellular localization
Secreted protein; extracellular space. -
Database links
- Entrez Gene: 6435 Human
- Entrez Gene: 6436 Human
- Omim: 178630 Human
- Omim: 178642 Human
- SwissProt: Q8IWL1 Human
- SwissProt: Q8IWL2 Human
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Alternative names
- 35 kDa pulmonary surfactant-associated protein antibody
- Alveolar proteinosis protein antibody
- COLEC4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)ab51891 staining sections of human lung at 1:3000 dilution. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
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![Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)](https://www.abcam.com/ps/products/51/ab51891/Images/ab51891-63958-anti-surfactant-protein-a-antibody-6f10-western-blot.jpg)
Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)All lanes : Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 secondsSurfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)](https://www.abcam.com/ps/products/51/ab51891/Images/ab51891-63957-anti-surfactant-protein-a-antibody-6f10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)ab51891 staining sections of human lung at 1:3000 dilution. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
Protocols
References (12)
ab51891 has been referenced in 12 publications.
- Liu Z et al. Surfactant protein A expression and distribution in human lung samples from smokers with or without chronic obstructive pulmonary disease in China. Medicine (Baltimore) 99:e19118 (2020). PubMed: 32049827
- Qian J et al. Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-?B pathway. Biosci Rep 40:N/A (2020). PubMed: 32915230
- Yin H et al. Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells. Oncogene 39:3754-3773 (2020). PubMed: 32157214
- Ezzat K et al. The viral protein corona directs viral pathogenesis and amyloid aggregation. Nat Commun 10:2331 (2019). PubMed: 31133680
- He X et al. Identification of a nanobody specific to human pulmonary surfactant protein A. Sci Rep 7:1412 (2017). IHC-Fr, ICC/IF ; Human . PubMed: 28469136
