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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial
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Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
  • Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)

Key features and details

  • Mouse monoclonal [6F10] to Surfactant Protein A/PSAP
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Surfactant Protein A/PSAP antibody [6F10]
    See all Surfactant Protein A/PSAP primary antibodies
  • Description

    Mouse monoclonal [6F10] to Surfactant Protein A/PSAP
  • Host species

    Mouse
  • Specificity

    ab51891 is specific for human pulmonary Surfactant Protein A/PSAP.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Human Surfactant Protein A/PSAP.

  • Positive control

    • Human lung tissue
  • General notes

     This product was previously labelled as Surfactant Protein A

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    6F10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (2)
1/1000. Predicted molecular weight: 26 kDa.
IHC-P
Use at an assay dependent concentration.
Notes
WB
1/1000. Predicted molecular weight: 26 kDa.
IHC-P
Use at an assay dependent concentration.

Target

  • Cellular localization

    Secreted protein; extracellular space.
  • Database links

    • Entrez Gene: 6435 Human
    • Entrez Gene: 6436 Human
    • Omim: 178630 Human
    • Omim: 178642 Human
    • SwissProt: Q8IWL1 Human
    • SwissProt: Q8IWL2 Human
    • Alternative names

      • 35 kDa pulmonary surfactant-associated protein antibody
      • Alveolar proteinosis protein antibody
      • COLEC4 antibody
      • Collectin 4 antibody
      • PSAP antibody
      • PSPA antibody
      • Pulmonary surfactant apoprotein antibody
      • Pulmonary surfactant-associated protein A1 antibody
      • SFTP1 antibody
      • SFTPA antibody
      • SFTPA1 antibody
      • SFTPA1B antibody
      • SP A antibody
      • SP A1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
    • Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      All lanes : Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891) at 1 µg/ml

      Lane 1 : Lung (Human) Tissue Lysate
      Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 26 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa, 63 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 150 seconds


      Surfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (12)

    Publishing research using ab51891? Please let us know so that we can cite the reference in this datasheet.

    ab51891 has been referenced in 12 publications.

    • Liu Z  et al. Surfactant protein A expression and distribution in human lung samples from smokers with or without chronic obstructive pulmonary disease in China. Medicine (Baltimore) 99:e19118 (2020). PubMed: 32049827
    • Qian J  et al. Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-?B pathway. Biosci Rep 40:N/A (2020). PubMed: 32915230
    • Yin H  et al. Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells. Oncogene 39:3754-3773 (2020). PubMed: 32157214
    • Ezzat K  et al. The viral protein corona directs viral pathogenesis and amyloid aggregation. Nat Commun 10:2331 (2019). PubMed: 31133680
    • He X  et al. Identification of a nanobody specific to human pulmonary surfactant protein A. Sci Rep 7:1412 (2017). IHC-Fr, ICC/IF ; Human . PubMed: 28469136
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Surfactant Protein A/PSAP antibody [6F10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RERF-LC-Ad1 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    RERF-LC-Ad1 cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2017

    Western blot abreview for Anti-Surfactant Protein A/PSAP antibody [6F10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (lung)
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Loading amount
    25 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Mun-Suk Park

    Verified customer

    Submitted Nov 01 2011

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