Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)

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Overview

  • Product name
    Anti-Surfactant Protein A/PSAP antibody [6F10]
    See all Surfactant Protein A/PSAP primary antibodies
  • Description
    Mouse monoclonal [6F10] to Surfactant Protein A/PSAP
  • Host species
    Mouse
  • Specificity

    ab51891 is specific for human pulmonary Surfactant Protein A/PSAP.

  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Surfactant Protein A/PSAP.

  • Positive control
    • Human lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab51891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21421565
WB 1/1000. Predicted molecular weight: 26 kDa.
ELISA 1/16000.
IHC-P Use at an assay dependent concentration.

Target

  • Cellular localization
    Secreted protein; extracellular space.
  • Database links
    • Alternative names
      • 35 kDa pulmonary surfactant-associated protein antibody
      • Alveolar proteinosis protein antibody
      • COLEC4 antibody
      • Collectin 4 antibody
      • PSAP antibody
      • PSPA antibody
      • Pulmonary surfactant apoprotein antibody
      • Pulmonary surfactant-associated protein A1 antibody
      • SFTP1 antibody
      • SFTPA antibody
      • SFTPA1 antibody
      • SFTPA1B antibody
      • SP A antibody
      • SP A1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
    • Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      All lanes : Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891) at 1 µg/ml

      Lane 1 : Lung (Human) Tissue Lysate
      Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 26 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa, 63 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 150 seconds


      Surfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
      ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.

    References

    This product has been referenced in:
    • He X  et al. Identification of a nanobody specific to human pulmonary surfactant protein A. Sci Rep 7:1412 (2017). IHC-Fr, ICC/IF ; Human . Read more (PubMed: 28469136) »
    • Nita I  et al. Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells. Sci Rep 7:41901 (2017). IF . Read more (PubMed: 28157203) »
    See all 8 Publications for this product

    Publishing research using ab51891? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Surfactant Protein A antibody [6F10]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RERF-LC-Ad1 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    RERF-LC-Ad1 cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2017

    Western blot abreview for Anti-Surfactant Protein A antibody [6F10]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (lung)
    Loading amount
    25 µg
    Specification
    lung
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Mun-Suk Park

    Verified customer

    Submitted Nov 01 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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