Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)](https://www.abcam.com/ps/products/51/ab51891/Images/ab51891-63959-anti-surfactant-protein-a-antibody-6f10-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)")
Key features and details
- Mouse monoclonal [6F10] to Surfactant Protein A/PSAP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Surfactant Protein A/PSAP antibody [6F10]
See all Surfactant Protein A/PSAP primary antibodies -
Description
Mouse monoclonal [6F10] to Surfactant Protein A/PSAP -
Host species
Mouse -
Specificity
ab51891 is specific for human pulmonary Surfactant Protein A/PSAP.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Surfactant Protein A/PSAP.
Database link: Q8IWL2 -
Positive control
- Human lung tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6F10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Predicted molecular weight: 26 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 26 kDa. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Cellular localization
Secreted protein; extracellular space. -
Database links
- Entrez Gene: 6435 Human
- Entrez Gene: 6436 Human
- Omim: 178630 Human
- Omim: 178642 Human
- SwissProt: Q8IWL1 Human
- SwissProt: Q8IWL2 Human
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Alternative names
- 35 kDa pulmonary surfactant-associated protein antibody
- Alveolar proteinosis protein antibody
- COLEC4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)ab51891 staining sections of human lung at 1:3000 dilution. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
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![Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)](https://www.abcam.com/ps/products/51/ab51891/Images/ab51891-63958-anti-surfactant-protein-a-antibody-6f10-western-blot.jpg)
Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)All lanes : Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 secondsSurfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)](https://www.abcam.com/ps/products/51/ab51891/Images/ab51891-63957-anti-surfactant-protein-a-antibody-6f10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)ab51891 staining sections of human lung at 1:3000 dilution. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab51891 has been referenced in 14 publications.
- Munis AM et al. A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications. Mol Ther Methods Clin Dev 20:237-246 (2021). PubMed: 33426150
- Liu Z et al. Surfactant protein A expression and distribution in human lung samples from smokers with or without chronic obstructive pulmonary disease in China. Medicine (Baltimore) 99:e19118 (2020). PubMed: 32049827
- Qian J et al. Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-?B pathway. Biosci Rep 40:N/A (2020). PubMed: 32915230
- Yin H et al. Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells. Oncogene 39:3754-3773 (2020). PubMed: 32157214
- Ezzat K et al. The viral protein corona directs viral pathogenesis and amyloid aggregation. Nat Commun 10:2331 (2019). PubMed: 31133680