Western blot abreview for Anti-Surfactant Protein A/PSAP antibody [6F10]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (RERF-LC-Ad1 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
RERF-LC-Ad1 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking agent
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-Linked Whole Ab Sheep
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 50, 30 kDa

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Submitted Nov 06 2017

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