Overview

  • Product name

    Anti-Surfactant Protein A/PSAP antibody
    See all Surfactant Protein A/PSAP primary antibodies
  • Description

    Rabbit polyclonal to Surfactant Protein A/PSAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human Surfactant Protein A/PSAP aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87829)

  • Positive control

    • This antibody gave a positive signal in Human Lung Tissue Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Rat lung.
  • General notes

     This product was previously labelled as Surfactant Protein A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab87674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • IHC image of Surfactant Protein A/PSAP staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Surfactant Protein A/PSAP antibody (ab87674) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    Surfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

ab87674 has not yet been referenced specifically in any publications.

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