Product nameAnti-Surfactant protein D / SP-D antibody [12G5]
See all Surfactant protein D / SP-D primary antibodies
DescriptionMouse monoclonal [12G5] to Surfactant protein D / SP-D
Tested applicationsSuitable for: IHC-Fr, ICC/IF, ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Cow, Human
Recombinant full length protein corresponding to Human Surfactant protein D/ SP-D.
- Epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder.
Previously labelled as Surfactant protein D.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 0.0268% PBS, 2.9% Sodium chloride
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab17781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/500. For 16 hrs on perinatal mouse lung samples (see Abreview for further details).|
|ELISA||Use at an assay dependent concentration.|
|WB||1/20000. Predicted molecular weight: 37 kDa.
ab17781 reacts strongly with Surfactant protein D / SP-D, both in reduced and non-reduced forms.
FunctionContributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Sequence similaritiesBelongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain.
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
- Information by UniProt
- COLEC 7 antibody
- COLEC7 antibody
- Collectin-7 antibody
ab17781 staining Surfactant protein D / SP-D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Counterstained with DAPI.
ab17781 staining Surfactant protein D / SP-D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.
ab17781 at a 1/500 dilution staining perinatal mouse lung tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2.5% BSA for 30 minutes. The antibody was incubated with the tissue for 16 hours and bound antibody was detected with an Alexa-Fluor ® 594 conjugated Goat anti-Mouse IgG antibody.
This image is courtesy of an Anonymous Abreview submitted on 3 April 2006.
This product has been referenced in:
- Bartolák-Suki E et al. Optimization of Variable Ventilation for Physiology, Immune Response and Surfactant Enhancement in Preterm Lambs. Front Physiol 8:425 (2017). Read more (PubMed: 28690548) »
- Kang X et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . Read more (PubMed: 25915125) »