Overview

  • Product name
    Anti-Surfactant protein D / SP-D antibody [12G5]
    See all Surfactant protein D / SP-D primary antibodies
  • Description
    Mouse monoclonal [12G5] to Surfactant protein D / SP-D
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Surfactant protein D/ SP-D.

  • Positive control
    • Epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder.
  • General notes

    Previously labelled as Surfactant protein D.

Properties

Applications

Our Abpromise guarantee covers the use of ab17781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500. For 16 hrs on perinatal mouse lung samples (see Abreview for further details).
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
WB 1/20000. Predicted molecular weight: 37 kDa.

ab17781 reacts strongly with Surfactant protein D / SP-D, both in reduced and non-reduced forms.

IHC-P 1/800.

Target

  • Function
    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similarities
    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links
  • Alternative names
    • COLEC 7 antibody
    • COLEC7 antibody
    • Collectin-7 antibody
    • Collectin7 antibody
    • Lung surfactant protein D antibody
    • PSP D antibody
    • PSP-D antibody
    • PSP-D Surfactant protein D antibody
    • PSPD antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant associated protein D antibody
    • Pulmonary surfactant associated protein PSP-D antibody
    • Pulmonary surfactant-associated protein D antibody
    • SFTP 4 antibody
    • SFTP4 antibody
    • SFTPD antibody
    • SFTPD_HUMAN antibody
    • SP D antibody
    • SP-D antibody
    • Surfactant associated protein pulmonary 4 antibody
    • Surfactant protein D antibody
    • Surfactant pulmonary associated protein D antibody
    see all

Images

  • ab17781 staining Surfactant protein D / SP-D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • ab17781 staining Surfactant protein D / SP-D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab17781 at a 1/500 dilution staining perinatal mouse lung tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2.5% BSA for 30 minutes. The antibody was incubated with the tissue for 16 hours and bound antibody was detected with an Alexa-Fluor ® 594 conjugated Goat anti-Mouse IgG antibody.

    This image is courtesy of an Anonymous Abreview submitted on 3 April 2006.

    See Abreview

References

This product has been referenced in:
  • Bartolák-Suki E  et al. Optimization of Variable Ventilation for Physiology, Immune Response and Surfactant Enhancement in Preterm Lambs. Front Physiol 8:425 (2017). Read more (PubMed: 28690548) »
  • Kang X  et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . Read more (PubMed: 25915125) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated amniotic epithelial cell)
Permeabilization
Yes - 0.25 % Triton X-100
Specification
differentiated amniotic epithelial cell
Blocking step
Roti Immunoblock as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde

Ms. Angela Lemke

Verified customer

Submitted Mar 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human gall bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
human gall bladder
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Mrs. Maria Cecilia Ybanez

Verified customer

Submitted Mar 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human gall bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
human gall bladder
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Steffen Rickelt

Verified customer

Submitted Mar 17 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (pancreatic ductal cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
pancreatic ductal cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Steffen Rickelt

Verified customer

Submitted Nov 04 2013

Question
Answer

Thank you for your phone call earlier today. I checked about the Surfactant protein D antibody you were looking for. We currently have several mouse monoclonal antibodies that may be suitable: ab17781, ab15696, ab15695 and ab15687 for example. I hope this helps, if you are looking for a different type of antibody please let me know and I will be happy to help you further.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (perinatal mouse lung)
Specification
perinatal mouse lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2.5%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 03 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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