Overview

  • Product name

    Anti-Surfactant protein D/SP-D antibody [12G5]
    See all Surfactant protein D/SP-D primary antibodies
  • Description

    Mouse monoclonal [12G5] to Surfactant protein D/SP-D
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Surfactant protein D/SP-D.

  • Positive control

    • Epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder.
  • General notes

    Previously labelled as Surfactant protein D.

Properties

Applications

Our Abpromise guarantee covers the use of ab17781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500. For 16 hrs on perinatal mouse lung samples (see Abreview for further details).
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
WB 1/20000. Predicted molecular weight: 37 kDa.

ab17781 reacts strongly with Surfactant protein D/SP-D, both in reduced and non-reduced forms.

IHC-P 1/800.

Target

  • Function

    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similarities

    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links

  • Alternative names

    • COLEC 7 antibody
    • COLEC7 antibody
    • Collectin-7 antibody
    • Collectin7 antibody
    • Lung surfactant protein D antibody
    • PSP D antibody
    • PSP-D antibody
    • PSP-D Surfactant protein D antibody
    • PSPD antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant associated protein D antibody
    • Pulmonary surfactant associated protein PSP-D antibody
    • Pulmonary surfactant-associated protein D antibody
    • SFTP 4 antibody
    • SFTP4 antibody
    • SFTPD antibody
    • SFTPD_HUMAN antibody
    • SP D antibody
    • SP-D antibody
    • Surfactant associated protein pulmonary 4 antibody
    • Surfactant protein D antibody
    • Surfactant pulmonary associated protein D antibody
    see all

Images

  • ab17781 staining Surfactant protein D/SP-D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • ab17781 staining Surfactant protein D/SP-D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab17781 at a 1/500 dilution staining perinatal mouse lung tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2.5% BSA for 30 minutes. The antibody was incubated with the tissue for 16 hours and bound antibody was detected with an Alexa-Fluor ® 594 conjugated Goat anti-Mouse IgG antibody.

    This image is courtesy of an Anonymous Abreview submitted on 3 April 2006.

    See Abreview

References

This product has been referenced in:

  • Bartolák-Suki E  et al. Optimization of Variable Ventilation for Physiology, Immune Response and Surfactant Enhancement in Preterm Lambs. Front Physiol 8:425 (2017). Read more (PubMed: 28690548) »
  • Sarashina-Kida H  et al. Gallbladder-derived surfactant protein D regulates gut commensal bacteria for maintaining intestinal homeostasis. Proc Natl Acad Sci U S A 114:10178-10183 (2017). Read more (PubMed: 28878025) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated amniotic epithelial cell)
Permeabilization
Yes - 0.25 % Triton X-100
Specification
differentiated amniotic epithelial cell
Blocking step
Roti Immunoblock as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde

Ms. Angela Lemke

Verified customer

Submitted Mar 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human gall bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
human gall bladder
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Mrs. Maria Cecilia Ybanez

Verified customer

Submitted Mar 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human gall bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
human gall bladder
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Steffen Rickelt

Verified customer

Submitted Mar 17 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (pancreatic ductal cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
pancreatic ductal cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Steffen Rickelt

Verified customer

Submitted Nov 04 2013

Question
Answer

Thank you for your phone call earlier today. I checked about the Surfactant protein D antibody you were looking for. We currently have several mouse monoclonal antibodies that may be suitable: ab17781, ab15696, ab15695 and ab15687 for example. I hope this helps, if you are looking for a different type of antibody please let me know and I will be happy to help you further.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (perinatal mouse lung)
Specification
perinatal mouse lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2.5%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 03 2006

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