For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    surfactant-protein-dsp-d-antibody-epr21774-153-ab220422.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email
RecombinantRabMAb

Recombinant Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21774-153] to Surfactant protein D/SP-D
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Recombinant fragment

You may also be interested in

Primary
Product image
Anti-NeuroD1 antibody [EPR4008] (ab109224)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Stain
Product image
Alcian Blue Stain Kit (pH 1.0, Mucin Stain) (ab150661)

View more associated products

Overview

  • Product name

    Anti-Surfactant protein D/SP-D antibody [EPR21774-153]
    See all Surfactant protein D/SP-D primary antibodies
  • Description

    Rabbit monoclonal [EPR21774-153] to Surfactant protein D/SP-D
  • Host species

    Rabbit
  • Specificity

    The Mouse species recommendation is based on the WB results. We do not guarantee IHC for Mouse.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    WB
    Mouse
    Rat
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse Surfactant protein D/SP-D recombinant protein (aa 1-374); Mouse lung tissue lysate; Rat lung tissue lysate. IHC-P: Rat lung tissue. IHC-Fr: Rat lung tissue.
  • General notes

    Previously labelled as Surfactant protein D.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21774-153
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-Surfactant protein D/SP-D antibody [EPR21774-153] - BSA and Azide free (ab234260)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Surfactant protein D/SP-D (ab152069)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab220422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-Fr
Rat
IHC-P
Rat
WB
Mouse
Rat
Recombinant fragment
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-P for Mouse.

IHC-Fr
1/500.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-F for Mouse.

Notes
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-P for Mouse.

IHC-Fr
1/500.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-F for Mouse.

Target

  • Function

    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similarities

    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Target information above from: UniProt accession P35247 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 20390 Mouse
    • Entrez Gene: 25350 Rat
    • SwissProt: P50404 Mouse
    • SwissProt: P35248 Rat
    • Unigene: 1321 Mouse
    • Unigene: 11348 Rat
    • Alternative names

      • COLEC 7 antibody
      • COLEC7 antibody
      • Collectin-7 antibody
      • Collectin7 antibody
      • Lung surfactant protein D antibody
      • PSP D antibody
      • PSP-D antibody
      • PSP-D Surfactant protein D antibody
      • PSPD antibody
      • Pulmonary surfactant apoprotein antibody
      • Pulmonary surfactant associated protein D antibody
      • Pulmonary surfactant associated protein PSP-D antibody
      • Pulmonary surfactant-associated protein D antibody
      • SFTP 4 antibody
      • SFTP4 antibody
      • SFTPD antibody
      • SFTPD_HUMAN antibody
      • SP D antibody
      • SP-D antibody
      • Surfactant associated protein pulmonary 4 antibody
      • Surfactant protein D antibody
      • Surfactant pulmonary associated protein D antibody
      see all

    Images

    • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422) at 1/1000 dilution + Mouse Surfactant protein D/SP-D recombinant protein (aa 1-374) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      Recombinant protein was obtained from a mammalian expression system.

    • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      All lanes : Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422) at 1/1000 dilution

      Lane 1 : Mouse lung tissue lysate
      Lane 2 : Rat lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times.

      Lane 1: 8 seconds.

      Lane 2: 26 seconds.

      The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757, PMID: 23390139).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Surfactant protein D/SP-D with ab220422 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in Clara cells and alveolar type II cells of rat lung (PMID: 10820266) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval using citrate buffer, pH 6.0.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

      Negative control: no staining on rat skeletal muscle (PMID: 10640760).

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220422 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval using citrate buffer, pH 6.0.

    • Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

      Immunohistochemical analysis of frozen sectioned 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat lung tissue labeling Surfactant protein D/SP-D with ab220422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in Clara cells and alveolar type II cells of rat lung (PMID: 2049389) is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

    • Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab220422? Please let us know so that we can cite the reference in this datasheet.

    ab220422 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab220422.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.