RecombinantRabMAb

Recombinant Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

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Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
  • Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21774-153] to Surfactant protein D/SP-D
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Recombinant fragment

Overview

  • Product name

    Anti-Surfactant protein D/SP-D antibody [EPR21774-153]
    See all Surfactant protein D/SP-D primary antibodies

  • Description

    Rabbit monoclonal [EPR21774-153] to Surfactant protein D/SP-D

  • Host species

    Rabbit

  • Specificity

    The Mouse species recommendation is based on the WB results. We do not guarantee IHC for Mouse.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    WB
    Mouse
    Rat
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse Surfactant protein D/SP-D recombinant protein (aa 1-374); Mouse lung tissue lysate; Rat lung tissue lysate. IHC-P: Rat lung tissue. IHC-Fr: Rat lung tissue.

  • General notes

    Previously labelled as Surfactant protein D.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab220422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-Fr
Rat
IHC-P
Rat
WB
Mouse
Rat
Recombinant fragment
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-P for Mouse.
IHC-Fr
1/500.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-F for Mouse.
Notes
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-P for Mouse.
IHC-Fr
1/500.

Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

The Mouse species recommendation is based on the WB results. We do not guarantee IHC-F for Mouse.

Target

  • Function

    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

  • Sequence similarities

    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.

  • Post-translational
    modifications

    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt

  • Database links

    • Alternative names

      • COLEC 7 antibody
      • COLEC7 antibody
      • Collectin-7 antibody
      • Collectin7 antibody
      • Lung surfactant protein D antibody
      • PSP D antibody
      • PSP-D antibody
      • PSP-D Surfactant protein D antibody
      • PSPD antibody
      • Pulmonary surfactant apoprotein antibody
      • Pulmonary surfactant associated protein D antibody
      • Pulmonary surfactant associated protein PSP-D antibody
      • Pulmonary surfactant-associated protein D antibody
      • SFTP 4 antibody
      • SFTP4 antibody
      • SFTPD antibody
      • SFTPD_HUMAN antibody
      • SP D antibody
      • SP-D antibody
      • Surfactant associated protein pulmonary 4 antibody
      • Surfactant protein D antibody
      • Surfactant pulmonary associated protein D antibody
      see all

    Images

    • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422) at 1/1000 dilution + Mouse Surfactant protein D/SP-D recombinant protein (aa 1-374) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      Recombinant protein was obtained from a mammalian expression system.

    • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      All lanes : Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422) at 1/1000 dilution

      Lane 1 : Mouse lung tissue lysate
      Lane 2 : Rat lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times.

      Lane 1: 8 seconds.

      Lane 2: 26 seconds.

      The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757, PMID: 23390139).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Surfactant protein D/SP-D with ab220422 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in Clara cells and alveolar type II cells of rat lung (PMID: 10820266) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval using citrate buffer, pH 6.0.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

      Negative control: no staining on rat skeletal muscle (PMID: 10640760).

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220422 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval using citrate buffer, pH 6.0.

    • Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

      Immunohistochemical analysis of frozen sectioned 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat lung tissue labeling Surfactant protein D/SP-D with ab220422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in Clara cells and alveolar type II cells of rat lung (PMID: 2049389) is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

    • Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
      Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab220422? Please let us know so that we can cite the reference in this datasheet.

    ab220422 has not yet been referenced specifically in any publications.

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