Recombinant Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21928-209] to Surfactant protein D/SP-D
- Suitable for: WB, IHC-P, IP, mIHC
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Surfactant protein D/SP-D antibody [EPR21928-209]
See all Surfactant protein D/SP-D primary antibodies -
Description
Rabbit monoclonal [EPR21928-209] to Surfactant protein D/SP-D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, mIHCmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human Surfactant protein D/SP-D recombinant protein (aa 21-375); Human lung lysate. IHC-P: Human lung and skeletal muscle tissue. IP: Human fetal lung lysate. mIHC-P: Human lung tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21928-209 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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mIHC |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
mIHC
Use at an assay dependent concentration. |
Target
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Function
Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. -
Sequence similarities
Belongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film. - Information by UniProt
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Database links
- Entrez Gene: 6441 Human
- Omim: 178635 Human
- SwissProt: P35247 Human
- Unigene: 253495 Human
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Alternative names
- COLEC 7 antibody
- COLEC7 antibody
- Collectin-7 antibody
see all
Images
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Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue.
Panel A: Merged staining of anti-Podoplanin / gp36 (magenta; Opal™690), anti-Surfactant protein D/SP-D (green; Opal™520) and anti-Sialoadhesin/CD169 (red; Opal™570) on human lung.
Panel B: Anti-Podoplanin / gp36 stained on alveolar type I cells.
Panel C: Anti-Surfactant protein D/SP-D stained on alveolar type II cells.
Panel D: Anti-Sialoadhesin/CD169 stained on macrophages.The section was incubated in three rounds of staining: in the order of ab236529 and ab234257 for 30 mins, then ab245735 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.
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Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423) at 1/1000 dilution + Human lung lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757).
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Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Surfactant protein D/SP-D with ab220423 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on alveolar type II cells of human lung (PMID: 10820266) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Negative control: No staining on human skeletal muscle (PMID: 10640760) is observed
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220423 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Surfactant protein D/SP-D was immunoprecipitated from 0.35 mg human fetal lung lysate with ab220423 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220423 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Human fetal lung lysate 10 µg (Input).
Lane 2: ab220423 IP in human fetal lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220423 in human fetal lung lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757).
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Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423) at 1/1000 dilution + Human Surfactant protein D/SP-D recombinant protein (aa 21-375), 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Exposure time: 32 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Recombinant protein was obtained from a mammalian expression system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab220423 has not yet been referenced specifically in any publications.