Recombinant
RabMAb

Recombinant Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

Overview

  • Product name

    Anti-Surfactant protein D/SP-D antibody [EPR21928-209]
    See all Surfactant protein D/SP-D primary antibodies
  • Description

    Rabbit monoclonal [EPR21928-209] to Surfactant protein D/SP-D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Surfactant protein D/SP-D aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P35247

  • Positive control

    • WB: Human Surfactant protein D/SP-D recombinant protein (aa 21-375); Human lung lysate. IHC-P: Human lung and skeletal muscle tissue. IP: Human fetal lung lysate.
  • General notes

    Previously labelled as Surfactant protein D.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab220423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similarities

    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links

  • Alternative names

    • COLEC 7 antibody
    • COLEC7 antibody
    • Collectin-7 antibody
    • Collectin7 antibody
    • Lung surfactant protein D antibody
    • PSP D antibody
    • PSP-D antibody
    • PSP-D Surfactant protein D antibody
    • PSPD antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant associated protein D antibody
    • Pulmonary surfactant associated protein PSP-D antibody
    • Pulmonary surfactant-associated protein D antibody
    • SFTP 4 antibody
    • SFTP4 antibody
    • SFTPD antibody
    • SFTPD_HUMAN antibody
    • SP D antibody
    • SP-D antibody
    • Surfactant associated protein pulmonary 4 antibody
    • Surfactant protein D antibody
    • Surfactant pulmonary associated protein D antibody
    see all

Images

  • Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423) at 1/1000 dilution + Human lung lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757).

  • Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Surfactant protein D/SP-D with ab220423 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on alveolar type II cells of human lung (PMID: 10820266) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Negative control: No staining on human skeletal muscle (PMID: 10640760) is observed

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220423 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Surfactant protein D/SP-D was immunoprecipitated from 0.35 mg human fetal lung lysate with ab220423 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220423 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: Human fetal lung lysate 10 µg (Input).
    Lane 2: ab220423 IP in human fetal lung lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220423 in human fetal lung lysate.
    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757).

  • Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423) at 1/1000 dilution + Human Surfactant protein D/SP-D recombinant protein (aa 21-375), 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa


    Exposure time: 32 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Recombinant protein was obtained from a mammalian expression system.

References

ab220423 has not yet been referenced specifically in any publications.

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