RecombinantRabMAb

Recombinant Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

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Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
  • Immunoprecipitation - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
  • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
  • Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21928-209] to Surfactant protein D/SP-D
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Surfactant protein D/SP-D antibody [EPR21928-209]
    See all Surfactant protein D/SP-D primary antibodies

  • Description

    Rabbit monoclonal [EPR21928-209] to Surfactant protein D/SP-D

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Surfactant protein D/SP-D aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P35247

  • Positive control

    • WB: Human Surfactant protein D/SP-D recombinant protein (aa 21-375); Human lung lysate. IHC-P: Human lung and skeletal muscle tissue. IP: Human fetal lung lysate.

  • General notes

    Previously labelled as Surfactant protein D.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab220423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

  • Sequence similarities

    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.

  • Post-translational
    modifications

    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt

  • Database links

    • Alternative names

      • COLEC 7 antibody
      • COLEC7 antibody
      • Collectin-7 antibody
      • Collectin7 antibody
      • Lung surfactant protein D antibody
      • PSP D antibody
      • PSP-D antibody
      • PSP-D Surfactant protein D antibody
      • PSPD antibody
      • Pulmonary surfactant apoprotein antibody
      • Pulmonary surfactant associated protein D antibody
      • Pulmonary surfactant associated protein PSP-D antibody
      • Pulmonary surfactant-associated protein D antibody
      • SFTP 4 antibody
      • SFTP4 antibody
      • SFTPD antibody
      • SFTPD_HUMAN antibody
      • SP D antibody
      • SP-D antibody
      • Surfactant associated protein pulmonary 4 antibody
      • Surfactant protein D antibody
      • Surfactant pulmonary associated protein D antibody
      see all

    Images

    • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423) at 1/1000 dilution + Human lung lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Surfactant protein D/SP-D with ab220423 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on alveolar type II cells of human lung (PMID: 10820266) is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

      Negative control: No staining on human skeletal muscle (PMID: 10640760) is observed

      Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220423 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunoprecipitation - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Immunoprecipitation - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

      Surfactant protein D/SP-D was immunoprecipitated from 0.35 mg human fetal lung lysate with ab220423 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220423 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
      Lane 1: Human fetal lung lysate 10 µg (Input).
      Lane 2: ab220423 IP in human fetal lung lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220423 in human fetal lung lysate.
      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

      The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757).

    • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423) at 1/1000 dilution + Human Surfactant protein D/SP-D recombinant protein (aa 21-375), 10 ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 38 kDa


      Exposure time: 32 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Recombinant protein was obtained from a mammalian expression system.

    • Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)
      Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (ab220423)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab220423? Please let us know so that we can cite the reference in this datasheet.

    ab220423 has not yet been referenced specifically in any publications.

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