Product nameAnti-Survivin 3 alpha antibody [9H18L32]
DescriptionRabbit monoclonal [9H18L32] to Survivin 3 alpha
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat, Dog, Pig, Orangutan
- MCF7 cells; HeLa, Jurkat, A431, HEK293 cell lysates; Mouse kidney, liver and testis tissue lysates; Rat kidney and testis tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- Mouse testis normal tissue lysate - total protein (ab29289)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A431 whole cell lysate (ab7909)
Our Abpromise guarantee covers the use of ab203571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 16 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||1/100 - 1/500.|
FunctionMultitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Sequence similaritiesBelongs to the IAP family.
Contains 1 BIR repeat.
Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
DomainThe BIR repeat is necessary and sufficient for LAMTOR5 binding.
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities.
Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.
- Information by UniProt
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
All lanes : Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 2 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : Mouse kidney tissue lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse testis tissue lysate
Lane 8 : Rat kidney tissue lysate
Lane 9 : Rat testis tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa
Immunofluorescent analysis of 4% parafolrmaldehyde fixed MCF7 cells labeling Survivin 3 alpha using ab203571 at 5 μg/ml. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). DAPI staining for nuclei (Blue).
ab203571 has not yet been referenced specifically in any publications.