Recombinant

Recombinant Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

Overview

  • Product name

    Anti-Survivin 3 alpha antibody [9H18L32]
  • Description

    Rabbit monoclonal [9H18L32] to Survivin 3 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cat, Dog, Pig, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human Survivin 3 alpha aa 1-142.
    Sequence:

    MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTEN EPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGE FLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD


    Database link: O15392

  • Positive control

    • MCF7 cells; HeLa, Jurkat, A431, HEK293 cell lysates; Mouse kidney, liver and testis tissue lysates; Rat kidney and testis tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    9H18L32
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab203571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 16 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt 1/100 - 1/500.

Target

  • Function

    Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificity

    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stage

    Expression is cell cycle-dependent and peaks at mitosis.
  • Domain

    The BIR repeat is necessary and sufficient for LAMTOR5 binding.
  • Post-translational
    modifications

    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities.
    Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.
  • Information by UniProt
  • Database links

  • Alternative names

    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • Survivin variant 3 alpha antibody
    see all

Images

  • All lanes : Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HEK293 cell lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Mouse liver tissue lysate
    Lane 7 : Mouse testis tissue lysate
    Lane 8 : Rat kidney tissue lysate
    Lane 9 : Rat testis tissue lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 16 kDa

  • Immunofluorescent analysis of 4% parafolrmaldehyde fixed MCF7 cells labeling Survivin 3 alpha using ab203571 at 5 μg/ml.  Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). DAPI staining for nuclei (Blue).

References

ab203571 has not yet been referenced specifically in any publications.

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